Stomata and Sporophytes of the Model Moss Physcomitrium patens

Frontiers in Plant Science
Robert S CaineJulie E Gray

Abstract

Mosses are an ancient land plant lineage and are therefore important in studying the evolution of plant developmental processes. Here, we describe stomatal development in the model moss species Physcomitrium patens (previously known as Physcomitrella patens) over the duration of sporophyte development. We dissect the molecular mechanisms guiding cell division and fate and highlight how stomatal function might vary under different environmental conditions. In contrast to the asymmetric entry divisions described in Arabidopsis thaliana, moss protodermal cells can enter the stomatal lineage directly by expanding into an oval shaped guard mother cell (GMC). We observed that when two early stage P. patens GMCs form adjacently, a spacing division can occur, leading to separation of the GMCs by an intervening epidermal spacer cell. We investigated whether orthologs of Arabidopsis stomatal development regulators are required for this spacing division. Our results indicated that bHLH transcription factors PpSMF1 and PpSCRM1 are required for GMC formation. Moreover, the ligand and receptor components PpEPF1 and PpTMM are also required for orientating cell divisions and preventing single or clustered early GMCs from developing adjacent to...Continue Reading

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Citations

Dec 8, 2020·Trends in Plant Science·Franziska FichtnerFrancois Barbier
Apr 21, 2021·Annals of Botany·Keiko U Torii
Aug 1, 2021·Current Opinion in Plant Biology·Roxane P Spiegelhalder, Michael T Raissig

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Methods Mentioned

BETA
Scanning electron microscopy
fluorescence imaging
Fluorescence
fluorescence microscopy

Software Mentioned

R Development Core Team
SPCH
PpTMM
ImageJ
ggplot2
MUTE
R

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