Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Liyong ZhangZhihua Liu

Abstract

Stomatin-like protein 2 (SLP-2) is a novel and unusual stomatin homologue of unknown functions. It has been implicated in interaction with erythrocyte cytoskeleton and presumably other integral membrane proteins, but not directly with the membrane bilayer. We show here the involvement of SLP-2 in human esophageal squamous cell carcinoma (ESCC), lung cancer, laryngeal cancer, and endometrial adenocarcinoma and the effects of SLP-2 on ESCC cells. Previous work of cDNA microarray in our laboratory revealed that SLP-2 was significantly up-regulated in ESCC. The expression of SLP-2 was further evaluated in human ESCC, lung cancer, laryngeal cancer, and endometrial adenocarcinoma by semiquantitative reverse transcription-PCR, Western blot, and immunohistochemistry. Mutation detection of SLP-2 exons was done by PCR and automated sequencing. Antisense SLP-2 eukaryotic expression plasmids were constructed and transfected into human ESCC cell line KYSE450. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, clonogenecity assay, flow cytometry assay, nude mice tumorigenetic assay, and cell attachment assay were done to investigate the roles of SLP-2 gene. All tumor types we tested showed overexpression of SLP-2 compared wi...Continue Reading

References

Mar 1, 1988·Computer Applications in the Biosciences : CABIOS·B A Jameson, H Wolf
Jul 15, 1994·International Journal of Cancer. Journal International Du Cancer·Y KandaM Fukumoto
Jun 21, 1996·International Journal of Cancer. Journal International Du Cancer·R MontesanoP Hainaut
Feb 3, 1998·International Journal of Epidemiology·J Chang-ClaudeJ Wahrendorf
Aug 17, 1999·International Journal of Cancer. Journal International Du Cancer·M RaidaK Höffken
Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·B S ChenM Wu
Feb 15, 2001·International Journal of Cancer. Journal International Du Cancer·J LuM Wu
Sep 20, 2002·Journal of the Association for Research in Otolaryngology : JARO·Bradley J GoldsteinRandall R Reed
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Huiying ZhiZhihua Liu
Jun 25, 2005·Journal of Wound Care·S L Briggs

❮ Previous
Next ❯

Citations

Feb 2, 2013·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Dongning LiuGuoxin Li
Jan 16, 2010·The Journal of Biological Chemistry·Yanyan TianZhihua Liu
Feb 13, 2008·BMC Evolutionary Biology·Jasper B Green, J Peter W Young
Feb 11, 2011·Journal of Experimental & Clinical Cancer Research : CR·Liangan ChenYanqin Li
Jun 10, 2010·World Journal of Gastroenterology : WJG·Lay-Chin YeohLay-Harn Gam
Aug 7, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei-Xin WangWei-Dong Qi
Oct 21, 2009·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Dong ChangTian-you Wang
Jan 12, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Aleksandar BartolomeDubravka Cvejic
Apr 20, 2011·European Journal of Cell Biology·Liudmilla LapatsinaGary R Lewin
Apr 7, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Anjian XuDacheng He
Aug 24, 2012·International Journal of Cancer. Journal International Du Cancer·Tatsuyuki TakadateMichiaki Unno
Jun 23, 2009·International Journal of Cancer. Journal International Du Cancer·Diana ZambelliKatia Scotlandi
Nov 7, 2006·Biochimica Et Biophysica Acta·Paul DowlingMartin Clynes
Dec 1, 2009·Cell Calcium·Sandrine Da CruzNicolas Demaurex
Aug 13, 2008·Biochimica Et Biophysica Acta·Vincent Soubannier, Heidi M McBride
Jun 16, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yan Li LiXing Xie
Feb 9, 2012·Journal of Proteome Research·Stephanie M PützAlbert Sickmann
Mar 11, 2018·The Journal of Biological Chemistry·Julie A GosneyBrian P Ceresa
Aug 17, 2018·Experimental and Therapeutic Medicine·Wenjing ChenJun Cheng
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark G KirchhofJoaquín Madrenas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.