Stomatin-like protein 2 is required for in vivo mitochondrial respiratory chain supercomplex formation and optimal cell function

Molecular and Cellular Biology
Panagiotis MitsopoulosJoaquín Madrenas

Abstract

Stomatin-like protein 2 (SLP-2) is a mainly mitochondrial protein that is widely expressed and is highly conserved across evolution. We have previously shown that SLP-2 binds the mitochondrial lipid cardiolipin and interacts with prohibitin-1 and -2 to form specialized membrane microdomains in the mitochondrial inner membrane, which are associated with optimal mitochondrial respiration. To determine how SLP-2 functions, we performed bioenergetic analysis of primary T cells from T cell-selective Slp-2 knockout mice under conditions that forced energy production to come almost exclusively from oxidative phosphorylation. These cells had a phenotype characterized by increased uncoupled mitochondrial respiration and decreased mitochondrial membrane potential. Since formation of mitochondrial respiratory chain supercomplexes (RCS) may correlate with more efficient electron transfer during oxidative phosphorylation, we hypothesized that the defect in mitochondrial respiration in SLP-2-deficient T cells was due to deficient RCS formation. We found that in the absence of SLP-2, T cells had decreased levels and activities of complex I-III2 and I-III2-IV(1-3) RCS but no defects in assembly of individual respiratory complexes. Impaired RCS...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E Bustamante, P L Pedersen
Apr 29, 1980·Biochemical and Biophysical Research Communications·M Fry, D E Green
Apr 25, 2000·The EMBO Journal·H Schägger, K Pfeiffer
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Leo G J NijtmansIan J Holt
Feb 20, 2003·Nature Biotechnology·Steven W TaylorSoumitra S Ghosh
Jul 17, 2003·The Journal of Biological Chemistry·Sandrine Da CruzJean-Claude Martinou
Oct 17, 2003·The Journal of Biological Chemistry·Kathy PfeifferHermann Schägger
Apr 1, 2004·Molecular Cell·Rebeca Acín-PérezJosé A Enríquez
Jun 23, 2004·The Journal of Biological Chemistry·Hermann SchäggerUlrich Brandt
Nov 5, 2004·Molecular Biology of the Cell·Takashi TatsutaThomas Langer
Jul 22, 2006·Journal of Molecular Biology·Matthew McKenzieMichael T Ryan
Feb 13, 2008·BMC Evolutionary Biology·Jasper B Green, J Peter W Young
Mar 15, 2008·Biochimica Et Biophysica Acta·Sandrine Da CruzJean-Claude Martinou
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark G KirchhofJoaquín Madrenas
Nov 26, 2008·Molecular Cell·Rebeca Acín-PérezJose Antonio Enriquez
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Feb 2, 2010·Biochimica Et Biophysica Acta·Giorgio LenazGiancarlo Solaini
Jan 12, 2011·The Journal of Cell Biology·Christof OsmanThomas Langer
Jun 18, 2011·Biochimica Et Biophysica Acta·Holger Seelert, Norbert A Dencher
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Jul 13, 2011·Molecular and Cellular Biology·Darah A ChristieJoaquín Madrenas
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Natalya V DudkinaEgbert J Boekema
Sep 13, 2011·The EMBO Journal·Thorsten AlthoffWerner Kühlbrandt
Mar 13, 2012·Cell Metabolism·Yu-Chan ChenJared Rutter
Oct 3, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Darah A ChristieJoaquín Madrenas
Feb 1, 2013·Journal of the American Chemical Society·Clement ArnarezXavier Periole
Jul 3, 2013·Science·Esther Lapuente-BrunJosé Antonio Enríquez
Jul 6, 2013·Protein & Cell·Rasika VartakYidong Bai
Oct 23, 2013·Methods in Cell Biology·Panagiotis Mitsopoulos, Joaquín Madrenas
Dec 26, 2013·Biochimica Et Biophysica Acta·Rebeca Acin-Perez, Jose A Enriquez
Mar 13, 2014·The Journal of Cell Biology·Ruchika AnandThomas Langer

❮ Previous
Next ❯

Citations

Feb 10, 2016·Trends in Biochemical Sciences·Sara CogliatiLuca Scorrano
Nov 8, 2015·Biochimica Et Biophysica Acta·Axel Magalon, François Alberge
Jan 13, 2016·Trends in Endocrinology and Metabolism : TEM·Timothy Wai, Thomas Langer
Oct 10, 2015·Cancer Letters·Sarah KoushyarD Alwyn Dart
Apr 24, 2016·Free Radical Biology & Medicine·Raquel Moreno-Loshuertos, José Antonio Enríquez
Apr 6, 2017·Cell Metabolism·Dusanka MilenkovicJudy Hirst
Jun 8, 2017·Journal of Proteome Research·Georg TascherFabrice Bertile
Feb 6, 2017·Cardiovascular Research·Jan Dudek, Christoph Maack
Jun 28, 2017·PloS One·Panagiotis MitsopoulosJoaquín Madrenas
Nov 10, 2016·Nature·Longyue L CaoNicholas E S Sibinga
Dec 7, 2018·Cells·Blanca Hernando-Rodríguez, Marta Artal-Sanz
Mar 2, 2019·Annual Review of Plant Biology·Etienne H MeyerChris Carrie
Oct 17, 2017·Frontiers in Cell and Developmental Biology·Jan Dudek
Nov 18, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sabzali JavadovAmadou Ks Camara
Jan 19, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Mauro SerricchioG Angus McQuibban
Mar 17, 2021·Free Radical Biology & Medicine·Pablo Hernansanz-Agustín, José Antonio Enríquez
Mar 17, 2021·Nature Chemical Biology·Christopher F BennettPere Puigserver
Apr 29, 2021·Journal of the American Society for Mass Spectrometry·Haorong LiLing Hao
May 27, 2021·Cell Reports·Alba Gonzalez-FranquesaAtul S Deshmukh
Jun 6, 2021·Heart, Lung & Circulation·Yuntao HuZhibing Qiu
Nov 9, 2021·Biochemical Society Transactions·Sara CogliatiJose Antonio Enriquez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.