Abstract
Microtubules assembled from pure tubulin in vitro are labile, rapidly depolymerized upon exposure to the cold. In contrast, in a number of cell types, cytoplasmic microtubules are stable, resistant to prolonged cold exposure. During the past years, the molecular basis of this microtubule stabilization in cells has been elucidated. Cold stability is due to polymer association with different variants of a calmodulin-regulated protein, STOP protein. The dynamic and hence the physiological consequences of STOP association with microtubules vary in different tissues. In neurons, STOP seems almost permanently associated with microtubules. STOP is apparently a major determinant of microtubule turnover in such cells and is required for normal neuronal differentiation. In cycling cells, only minor amounts of STOP are associated with interphase microtubules and STOP does not measurably affects microtubule dynamics. However, STOP is associated with mitotic microtubules in the spindle. Recent results indicate that such an association could be vital for meiosis and for the long-term fidelity of the mitotic process.
References
Jan 1, 1979·Cell Biology International Reports·A D BershadskyI S Tint
Jan 1, 1978·Cell·R L Margolis, L Wilson
Feb 1, 1990·Nature·S Okabe, N Hirokawa
Oct 29, 1991·Biochemistry·L Paturle-LafanechèreD Job
Aug 10, 1990·Cell·L D BelmontT J Mitchison
Jan 24, 1989·Biochemistry·F PirolletR L Margolis
Jul 1, 1989·The Journal of Cell Biology·S S LimG G Borisy
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L MargolisD Job
Sep 1, 1986·The Journal of Cell Biology·P W Baas, S R Heidemann
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Mar 19, 1984·Brain Research·M M BlackJ T Kurdyla
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D JobR L Margolis
Feb 2, 1982·Biochemistry·D JobR L Margolis
Jan 1, 1982·The Journal of Cell Biology·J R Morris, R J Lasek
Apr 29, 1980·Biochemistry·B C Webb, L Wilson
Jan 1, 1980·Cell and Tissue Research·S MoskalewskiU Friberg
Jun 1, 1995·Neuron·S TakedaN Hirokawa
Mar 1, 1994·The Journal of Cell Biology·A LieuvinD Job
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C BoscR L Margolis
Feb 1, 1996·Current Opinion in Cell Biology·F J McNally
Mar 1, 1997·European Journal of Biochemistry·T H MacRae
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·E DenarierD Job
Jul 14, 1998·The Journal of Cell Biology·L GuillaudD Job
Citations
Mar 31, 2007·Journal of Neuroscience Research·Alice CouégnasNelly Boehm
Dec 8, 2011·Journal of Neurochemistry·Patrick Schloss, Thorsten Lau
Jul 21, 2009·Protist·Antonio Pereira-Neves, Marlene Benchimol
Oct 20, 2006·Journal of Psychopharmacology·Sharon L EastwoodPaul J Harrison
Oct 27, 2011·Anti-cancer Drugs·Luis Miguel Anton AparicioGuadalupe Aparicio Gallego
Feb 13, 2015·Journal of Cell Science·Denis DacheuxMélanie Bonhivers