Storage conditions modulate the metabolomic profile of a black raspberry nectar with minimal impact on bioactivity

Food & Function
Matthew D TeegardenDevin G Peterson

Abstract

Pre-clinical and clinical studies suggest black raspberries (BRBs) may inhibit the development of oral cancer. Lyophilized BRB powder is commonly used in these studies, but processed BRB products are more often consumed. The objective of this work was to understand how storage conditions influence the phytochemical profile and anti-proliferative activity of a BRB nectar beverage. Untargeted UHPLC-Q-TOF-MS based metabolomics analyses demonstrated that large chemical variation was introduced by storage above -20 °C over 60 days. However, minimal change in anti-proliferative activity was observed when stored nectar extracts were applied to SCC-83-01-82 premalignant oral epithelial cells. As proof of concept, cyanidin-3-O-rutinoside and its degradation product, protocatechuic acid, were administered in different ratios maintaining an equimolar dose, and anti-proliferative activity was maintained. This study shows the utility of metabolomics to profile global chemical changes in foods, while demonstrating that isolated phytochemicals do not explain the complete bioactivity of a complex food product.

References

Feb 17, 2005·Journal of Agricultural and Food Chemistry·Addie A van der SluisMartinus A J S van Boekel
May 12, 2005·Journal of Agricultural and Food Chemistry·Vandana M Totlani, Devin G Peterson
Mar 14, 2007·Journal of Agricultural and Food Chemistry·Anita SrivastavaGerard Krewer
Mar 27, 2007·The British Journal of Nutrition·E Katherine KemsleyIan J Colquhoun
Jan 24, 2008·Journal of Agricultural and Food Chemistry·Yanjun ZhangDavid Heber
Feb 23, 2008·Journal of Agricultural and Food Chemistry·Artemio Z TulioA Raymond Miller
Jan 14, 2009·Cancer Prevention Research·Li-Shu WangGary D Stoner
Feb 14, 2009·Journal of Agricultural and Food Chemistry·Cindi BrownmillerRonald L Prior
May 6, 2009·Molecular Nutrition & Food Research·Colin D KayAedin Cassidy
Sep 19, 2009·Molecular Nutrition & Food Research·Claudine ManachAugustin Scalbert
Jan 17, 2012·Journal of Agricultural and Food Chemistry·Luke R HowardJackson O Lay
Nov 20, 2012·Nucleic Acids Research·David S WishartAugustin Scalbert
Sep 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Lloyd W SumnerMark R Viant
Oct 10, 2013·Database : the Journal of Biological Databases and Curation·Joseph A RothwellAugustin Scalbert
Dec 19, 2013·Journal of Agricultural and Food Chemistry·Junnan GuYael Vodovotz
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Dec 3, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Blake M WarnerChristopher M Weghorst
Dec 25, 2015·Nutrition and Cancer·Marta Kula, Mirosława Krauze-Baranowska
Sep 7, 2016·Journal of Berry Research·Laura A KrestyGary D Stoner
Jan 18, 2017·Chemical Research in Toxicology·Karam El-BayoumyJoseph B Guttenplan
May 20, 2017·Journal of Agricultural and Food Chemistry·Ian RonningenDevin G Peterson
Dec 20, 2017·Journal of Agricultural and Food Chemistry·Ian G Ronningen, Devin G Peterson

❮ Previous
Next ❯

Methods Mentioned

BETA
feature extraction
Profiler
PCA

Software Mentioned

ropls
SAS
Mass Profiler Professional
Profinder
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.