Storage of leukocyte-poor red cell concentrates: filtration in a closed system using a sterile connection device

Vox Sanguinis
R N PieterszJ A Loos

Abstract

Storage of leukocyte-poor red cell concentrates (LP-RCC) was investigated after filtration in a closed system that was assembled using a Sterile Connection Device (SCD). The LP-RCC were stored for up to 6 weeks following filtration with either 0.9% saline solution (n = 14) or saline-adenine-glucose-mannitol (SAG M) solution (n = 15) to prime and rinse the cellulose acetate filter. The results were compared with the data of nonfiltered buffy-coat-poor red cell concentrates (BC-poor RCC) stored in SAG M solution (n = 10). All LP-RCC contained less than 10(6) leukocytes whereas the nonfiltered BC-poor RCC contained 675 +/- 286 X 10(6) leukocytes at day 1, decreasing to 83 +/- 49 x 10(6) at day 42. Although glucose consumption, lactic acid production and decrease in pH was similar from day 7 through 28 in both groups of LP-RCC, a significantly steeper decline of ATP values as well as a higher hemolysis and LDH release was observed in the LP-RCC filtered with saline. During storage of the nonfiltered BC-poor RCC in SAG M, significantly higher glucose consumption (p less than 0.01), LDH release (p less than 0.001), rate of hemolysis (p less than 0.001) and a lower pH (p less than 0.001) were found, compared to the filtered units. It ...Continue Reading

References

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Citations

Jun 3, 1999·Transfusion Science·R N PieterszM J Seghatchian
May 13, 1998·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J A Loos, J L Wautier
Sep 28, 2002·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Ralf Karger, Volker Kretschmer
Jun 24, 2000·Transfusion·P F van Der MeerH W Reesink
Jun 1, 1994·British Journal of Haematology·W A HeatonE Nelson
Jan 1, 1994·Vox Sanguinis·A M Joustra-DijkhuisH W Reesink
May 15, 2007·Chest·Paul C HébertHoward L Corwin
Jan 1, 1993·Transfusion Medicine Reviews·R N PieterszH W Reesink
Nov 2, 2006·Transfusion·Alan TinmouthUNKNOWN Canadian Critical Care Trials Group

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