Storage of whole blood for up to 24 hours at ambient temperature prior to component preparation

Vox Sanguinis
R N PieterszJ A Loos

Abstract

The effect of rapid cooling to 20-24 degrees C of whole blood immediately after collection, using 'cooling units' with butane-1,4-diol and prolonged storage up to 24 h at ambient temperature was investigated in the whole blood and the subsequently prepared plasma, buffy coat and buffy-coat-poor red cell concentrate (BC-poor RCC) in saline-adenine-glucose-mannitol (SAG M) solution. Factor VIII:C content of the plasma (n = 10), after 24 h storage was 80 +/- 3% of the initial value. In routine procedures factor VIII:C content in the plasma (n = 129 pools of 20 donor units plasma) was 0.77 +/- 0.078 IU/ml, after storage of the whole blood for 16-20 h. In whole blood (n = 10), the 2,3-diphosphoglycerate (2,3-DPG) content of the red cells decreased from 4.36 +/- 0.55 to 1.47 +/- 0.6 mumol/ml red cells after 24 h storage at 20-24 degrees C. After storage of the BC-poor RCC (n = 10) at 2-6 degrees C for 1 week, the 2,3-DPG had dropped to 0.76 +/- 0.46 mumol/ml red cells. During the first 24 h of storage of whole blood, the adenine triphosphate (ATP) levels of the red cells remained stable. A mean increase of 20% of the initial value was observed after addition of SAG M solution. In the BC-poor RCC the ATP slowly decreased to 81 +/- 5% ...Continue Reading

References

Oct 1, 1976·Scandinavian Journal of Clinical and Laboratory Investigation·J H StrömmeL Eldjarn
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Mar 1, 1970·Journal of Clinical Pathology·P P Anthony, R A Drury

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Citations

Nov 3, 1997·Transfusion Science·R StampfliU E Nydegger
May 13, 1998·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J A Loos, J L Wautier
Sep 6, 2000·Vox Sanguinis·P F van der MeerH W Reesink
Apr 12, 2005·Transfusion·Scott Murphy
Jan 1, 1994·Vox Sanguinis·A M Joustra-DijkhuisH W Reesink
Aug 15, 1998·Vox Sanguinis·C F Högman
Aug 15, 1998·Vox Sanguinis·G Myllylä
Aug 1, 1996·Intensive Care Medicine·H M Oudemans-van StraatenC P Stoutenbeek
Jan 1, 1995·Immunological Investigations·W A Heaton

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