Store-operated Orai1 and IP3 receptor-operated TRPC1 channel.

Channels
Vladislav ZarayskiyVictoria M Bolotina

Abstract

Store-operated channels (SOC) are known to be physiologically activated following agonist-induced IP3 production and depletion of Ca2+ stores. Here we present molecular,biophysical and mechanistic evidence that two ubiquitously expressed plasma membrane channels may be responsible for creating a complex and sometimes controversial SOC image: one being a real SOC encoded by Orai1 and activated exclusively upon depletion of Ca2+ stores (via iPLA2beta -dependent pathway), while the second one is an IP3 receptor-operated channel (IP3ROC) encoded by TRPC1 and activated via its conformational coupling with IP3 receptor. In RBL-2H3 cells endogenously expressing Orai1 and TRPC1, we unmasked and characterized whole-cell current through IP3ROC channels that was hiding behind some familiar fingerprints of ICRAC, a current through the classical Ca2+-selective SOC (CRAC) channels. We discriminated these currents by their molecular identity, selectivity and different requirements for store depletion, IP3, iPLA2beta and conformational coupling to IP3 receptor. New knowledge on the properties and coexistence of Orai1-encoded SOC and TRPC1-encoded IP3ROC, and the use of experimental approaches introduced in this manuscript should help avoid fur...Continue Reading

Citations

Dec 25, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Kristen M ParkVictoria M Bolotina
May 24, 2008·The Journal of Physiology·Victoria M Bolotina
Sep 9, 2011·IUBMB Life·Claudia Moreno, Luis Vaca
Apr 1, 2009·The Journal of Physiology·Wayne I DeHavenJames W Putney
Sep 3, 2010·Journal of Cellular and Molecular Medicine·Jeremy T SmythJames W Putney
Jun 8, 2012·The Journal of Physiology·Tünde MolnarDavid Križaj
Jan 13, 2016·Nature Communications·Qingde ZhouVictoria M Bolotina
Sep 5, 2008·American Journal of Physiology. Cell Physiology·Tomasz GwozdzVictoria M Bolotina
Sep 17, 2009·Immunological Reviews·James W Putney
Apr 15, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marie PotierMohamed Trebak
Feb 28, 2020·World Journal of Gastrointestinal Oncology·Wen-Qi CuiLi-Xuan Sang
Apr 7, 2009·Cell Calcium·Damian B van Rossum, Randen L Patterson

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