Strain-dependent interactions of Streptococcus gallolyticus subsp. gallolyticus with human blood cells

BMC Microbiology
Imke GrimmTanja Vollmer

Abstract

Streptococcus gallolyticus subsp. gallolyticus (S. gallolyticus) is the causative pathogen in up to 20% of streptococcal-induced infective endocarditis (IE) cases. However, the underlying mechanisms of pathogenesis in S. gallolyticus have not yet been solved. Pathogens causing IE need to employ virulent strategies to initiate and establish infections, such as escape the bloodstream, invade the host-cell, and persist intracellularly. In this study, we examined the induction of inflammation by different S. gallolyticus strains in relation to their survival in whole blood and cell culture models as well as their ability to induce platelet aggregation. Phagocytosis of these bacteria by macrophages, followed by intracellular survival, was also quantified. In whole blood and THP-1 cell culture assays bacterial growth kinetics was determined by plating, followed by colony counting. Induction of interleukin (IL)-6 expression in whole blood of three healthy volunteers, caused by different strains, was quantified by ELISA. Gene expression of cytokines (IL1B, IL6 and IL8) was quantified by real-time PCR after stimulating THP-1 monocytes with bacteria. Induction of platelet aggregation was analyzed by light transmission aggregometry using ...Continue Reading

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Citations

May 26, 2018·Future Microbiology·Imke Grimm, Tanja Vollmer

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
flow cytometry
FCS
PCR
PMA
Assay

Software Mentioned

Apact
GraphPad
GraphPad Prism

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