Strain-dependent regulation of neurotransmission and actin-remodelling proteins in the mouse hippocampus

Genes, Brain, and Behavior
Daniela D PollakGert Lubec

Abstract

Individual mouse strains differ significantly in terms of behaviour, cognitive function and long-term potentiation. Hippocampal gene expression profiling of eight different mouse strains points towards strain-specific regulation of genes involved in neuronal information storage. Protein expression with regard to strain- dependent expression of structures related to neuronal information storage has not been investigated yet. Herein, a proteomic approach based on two-dimensional gel electrophoresis coupled with mass spectrometry (MALDI-TOF/TOF) has been chosen to address this question by determining strain-dependent expression of proteins involved in neurotransmission and activity-induced actin remodelling in hippocampal tissue of five mouse strains. Of 31 spots representing 16 different gene products analysed and quantified, N-ethylmaleimide-sensitive fusion protein, N-ethylmaleimide-sensitive factor attachment protein-alpha, actin-like protein 3, profilin and cofilin were expressed in a strain-dependent manner. By treating protein expression as a phenotype, we have shown significant genetic variation in brain protein expression. Further experiments in this direction may provide an indication of the genetic elements that contrib...Continue Reading

References

Jun 6, 2000·Learning & Memory·P V NguyenR Bourtchouladze
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Mar 26, 2002·Nature Genetics·Joachim KloseHans Lehrach
Jun 28, 2003·Analytical and Bioanalytical Chemistry·Detlev SuckauArmin Holle
May 3, 2005·Neurochemistry International·Joo-Ho ShinGert Lubec

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Citations

Jul 31, 2013·Genomics, Proteomics & Bioinformatics·Tristan D McClure-BegleyMarina R Picciotto
Jul 29, 2006·The Journal of Biological Chemistry·Victor RomanenkoTed Begenisich

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