Abstract
Bordetella pertussis, the causative agent of whooping cough, expresses many virulence factors believed to be involved in infection and disease progression. While these factors as a group are required for infection, deletion of individual virulence factor genes generally has limited effects on the ability of B. pertussis to efficiently infect the respiratory tract of mice, suggesting they may perform noncritical or redundant functions. We have recently observed that a B. pertussis strain, putatively with a mutation of a single gene, brkA, results in a severe defect in vivo. Although BrkA has been shown to be required for B. pertussis to resist complement-mediated killing in vitro, the relevance of these findings to the in vivo role of BrkA during infection has not been examined. Transducing this mutation into multiple wild-type B. pertussis strains allowed us to confirm the in vitro phenotype of reduced resistance to serum complement. All DeltabrkA mutants were increased in their sensitivity to complement in vitro, both in the presence and absence of antibodies. However, these strains differed substantially in their phenotypes in vivo. DeltabrkA mutants of recent clinical isolates were indistinguishable from wild-type strains in...Continue Reading
References
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I G CharlesN F Fairweather
Sep 1, 1989·Infection and Immunity·C A EwanowichM S Peppler
Dec 1, 1989·Infection and Immunity·A A Weiss, M S Goodwin
Jan 1, 1986·Annual Review of Microbiology·A A Weiss, E L Hewlett
Aug 1, 1988·Journal of General Microbiology·M A McLaffertyE L Hewlett
Aug 15, 1970·Nature·U K Laemmli
Oct 1, 1983·Infection and Immunity·A A WeissS Falkow
Nov 1, 1994·Infection and Immunity·R C Fernandez, A A Weiss
Aug 1, 1994·Infection and Immunity·P A Cotter, J F Miller
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·R C Fernandez, A A Weiss
Dec 1, 1996·Molecular Microbiology·G Martínez de TejadaP A Cotter
May 29, 1998·Infection and Immunity·G Martinez de TejadaJ F Miller
Jun 19, 1998·FEMS Microbiology Letters·R C Fernandez, A A Weiss
Mar 18, 1999·FEMS Microbiology Letters·B N Passerini de RossiJ P Rossi
Jul 17, 1999·Immunopharmacology·A CircoloH R Colten
Jul 23, 1999·Infection and Immunity·A PrestonD J Maskell
Sep 11, 1999·Journal of Bacteriology·J L Shannon, R C Fernandez
Oct 26, 1999·Infection and Immunity·E T HarvillJ F Miller
Nov 18, 2000·Infection and Immunity·E T HarvillJ F Miller
Feb 15, 2001·Current Opinion in Microbiology·C LochtF Jacob-Dubuisson
Apr 9, 2001·Infection and Immunity·M G Barnes, A A Weiss
Nov 2, 2001·Frontiers in Bioscience : a Journal and Virtual Library·S MattooJ F Miller
Dec 24, 2002·Infection and Immunity·Valorie C BurnsEric T Harvill
Jan 4, 2003·Journal of Bacteriology·David C OliverRachel C Fernandez
Mar 26, 2003·Infection and Immunity·Girish S KirimanjeswaraEric T Harvill
Aug 23, 2003·Infection and Immunity·Elizabeth J PishkoEric T Harvill
Citations
Jul 18, 2012·Infection and Immunity·Jonathan D LenzVirginia L Miller
Oct 24, 2007·Infection and Immunity·Si Ying HoSylvie Alonso
Jul 15, 2009·Infection and Immunity·Anne M BuboltzEric T Harvill
Sep 7, 2006·Journal of Clinical Microbiology·Paola StefanelliPaola Mastrantonio
Dec 21, 2010·PloS One·Yong-Zhang ZhuXiao-Kui Guo
May 11, 2011·Microbial Pathogenesis·Mojtaba NoofeliRoger Parton
Jun 28, 2008·Vaccine·Nico MarrRachel C Fernandez
Jan 21, 2014·Cellular Microbiology·Tridib GangulyRajendar Deora
May 10, 2013·Journal of Medical Microbiology·M Chelsea LaneVirginia L Miller
Feb 11, 2011·The Biochemical Journal·Yujia ZhaiFei Sun
Dec 21, 2010·Molecular Immunology·Hanne AmdahlSeppo Meri
Apr 28, 2012·Journal of Microbiological Methods·Yulanda M WilliamsonJohn R Barr
Jan 1, 2011·Results in Immunology·Ashutosh K PathakIsabella M Cattadori
Mar 18, 2016·PloS One·Gianpiero D PalermoZev Rosenwaks
Mar 22, 2018·Emerging Microbes & Infections·Noha M Elhosseiny, Ahmed S Attia
Dec 6, 2017·Molecular & Cellular Proteomics : MCP·Gianmarco GasperiniRosanna Leuzzi
Aug 2, 2014·Expert Review of Vaccines·Jeroen GeurtsenGermie P J M van den Dobbelsteen
May 15, 2007·Clinical and Vaccine Immunology : CVI·Jeroen GeurtsenRob J Vandebriel
Jan 1, 2020·Scientific Reports·Monica C GestalEric T Harvill
May 24, 2019·MSphere·Laura A GonyarJoshua C Eby
May 20, 2020·Frontiers in Microbiology·Anaïs ThiriardCamille Locht