Strain differentiation of Trichophyton rubrum by randomly amplified polymorphic DNA and analysis of rDNA nontranscribed spacer

Journal of Medical Microbiology
Lilian Cristiane BaezaMaria José Soares Mendes-Giannini

Abstract

Trichophyton rubrum is the most common pathogen causing dermatophytosis. Molecular strain-typing methods have recently been developed to tackle epidemiological questions and the problem of relapse following treatment. A total of 67 strains of T. rubrum were screened for genetic variation by randomly amplified polymorphic DNA (RAPD) analysis, with two primers, 5'-d[GGTGCGGGAA]-3' and 5'-d[CCCGTCAGCA]-3', as well as by subrepeat element analysis of the nontranscribed spacer of rDNA, using the repetitive subelements TRS-1 and TRS-2. A total of 12 individual patterns were recognized with the first primer and 11 with the second. Phylogenetic analysis of the RAPD products showed a high degree of similarity (>90 %) among the epidemiologically related clinical isolates, while the other strains possessed 60 % similarity. Specific amplification of TRS-1 produced three strain-characteristic banding patterns (PCR types); simple patterns representing one copy of TRS-1 and two copies of TRS-2 accounted for around 85 % of all isolates. It is concluded that molecular analysis has important implications for epidemiological studies, and RAPD analysis is especially suitable for molecular typing in T. rubrum.

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Citations

Mar 19, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A Hryncewicz-GwóźdźE Baran
Feb 16, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M F SpessoL S Chiapello
Nov 13, 2009·BMC Infectious Diseases·Beata KrawczykJózef Kur
Mar 26, 2013·Enfermedades infecciosas y microbiología clínica·Leonel PereiraNelson Lima
Mar 3, 2007·International Journal of Antimicrobial Agents·Daniel de Assis SantosPatrícia Silva Cisalpino
Sep 16, 2011·Mycoses·Anita Hryncewicz-GwóźdźJacek C Szepietowski
Mar 5, 2015·The Journal of Dermatology·Takashi MochizukiKazushi Anzawa
Jun 3, 2016·Medical Mycology Journal·Kiminobu TakedaTakashi Mochizuki
Sep 26, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Claudia CafarchiaDomenico Otranto
Mar 27, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Marcelo Teruyuki MatsumotoMaria José Soares Medes-Giannini
Sep 4, 2018·Japanese Journal of Infectious Diseases·Shusaku SuzukiKatsumi Fujitani
Feb 17, 2017·Medical Mycology·Ivan M PchelinAnastasia E Taraskina
May 15, 2008·Mycopathologia·Susan M Abdel-Rahman
May 16, 2008·Mycopathologia·Toshio Kanbe
Aug 19, 2020·Journal of the American Podiatric Medical Association·Aditya K Gupta, Kerry-Ann Nakrieko
Jul 11, 2014·Indian Journal of Medical Microbiology·E ElavarashiR Sudha

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