PMID: 3755637Jul 1, 1986Paper

Strain-specific differences in levels of the mRNA for the epinephrine synthesizing enzyme phenylethanolamine N-methyltransferase

Brain Research
M J EvingerT H Joh

Abstract

The activity of the epinephrine biosynthetic enzyme phenylethanolamine N-methyltransferase (PNMT, EC 2.1.1.28) is 3- to 8-fold greater in rats of the Fischer 344 and Buffalo strains. The biochemical basis for the strain differences has been analyzed at the level of PNMT protein and messenger RNA production. Fischer rat adrenals possess approximately 5-fold more PNMT protein than those of the Buffalo rat as established by Western blotting and immunoprecipitation of adrenal gland homogenates. Poly(A)+ RNAs purified from adrenal glands of each strain were translated in a reticulocyte lysate system, immunoprecipitated with antibody to PNMT and fractionated by SDS-PAGE. A 35S-labelled protein of Mr = 34,000 was immunoprecipitated from adrenals of Fischer and Buffalo rats, indicating that the molecular weights of PNMT do not differ in these strains prior to post-translational processing. Hybridization of a 740 base pair (bp) cDNA for PNMT indicated that the mRNAs for PNMT are the same size in the adrenals of both strains. However, the adrenals of Fischer rats contain 2- to 4-fold more PNMT mRNA than Buffalo rats, as established by quantitative dot blot hybridization and Northern blot analysis. The medulla oblongata, the site of cell ...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WahlG R Stark
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E E BaetgeT H Joh
Feb 1, 1972·Behavioral Biology·D S SegalA J Mandell
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jun 4, 1974·Biochemistry·V GlisinC Byus
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E E BaetgeT H Joh
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·T H JohD J Reis
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1982·Annual Review of Pharmacology and Toxicology·R W Fuller
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E SabbanI B Black

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Citations

Jun 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B HansenR Westermann
Apr 18, 2002·Journal of Neuroimmunology·Michael B MannDavid E Comings

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