Strain-specific polyketide synthase genes of Aspergillus niger

International Journal of Food Microbiology
Lara Munique FerracinMaria Helena Pelegrinelli Fungaro

Abstract

In silico comparison of 34 putative pks genes in Aspergillus niger strain CBS 513.88 versus A. niger strain ATCC 1015 genome revealed significant nucleotide identity (>95% covering a minimum of 99% of the gene sequence) for 31 of these genes (approximately 91%). A. niger CBS 513.88 harbors three putative pks genes (An01g01130, An11g05940, and An15g07920), for which nucleotide identity was not found in A. niger ATCC 1015. To compare the results of the in silico analysis with the in vivo situation, experimental data were obtained for a large number of A. niger strains obtained from different substrates and geographical regions. Three putative pks genes that were found to be variable between the two A. niger strains using bioinformatics tools were in fact strain-specific genes based on experimental data. The PCR amplification signals for the An01g01130, An11g05940, and An15g07920 pks genes were detected in only 97%, 71%, and 26% of the strains, respectively. Southern blot analyses confirmed the PCR data. Because one of the strain-specific pks genes (An15g07920) is located in a putative ochratoxin cluster, we focused our investigation on that region. We assessed the ochratoxin production capability of the 119 A. niger strains and f...Continue Reading

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Citations

Jul 31, 2012·Evolutionary Bioinformatics Online·Shu-Hsi LinMasanori Arita
Jan 25, 2016·International Journal of Food Microbiology·Fernanda Pelisson MassiMaria Helena Pelegrinelli Fungaro
Apr 10, 2016·International Journal of Food Microbiology·Massimo FerraraAntonia Susca
Jul 17, 2016·Applied and Environmental Microbiology·Massimo FerraraAntonia Gallo
Apr 1, 2014·Journal of Food Protection·Nam Yeun KimGeun Eog Ji
Dec 26, 2018·International Journal of Food Microbiology·Bárbara Alves Dos Santos-CisconCees Waalwijk
Oct 24, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Fernanda Pelisson MassiMaria Helena Pelegrinelli Fungaro
Nov 3, 2020·Food Science & Nutrition·Youssuf A GherbawyEman G A El-Dawy
May 31, 2021·Mycopathologia·Mohammad Javad NajafzadehJos Houbraken

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.