Strain waveform dependence of stress fiber reorientation in cyclically stretched osteoblastic cells: effects of viscoelastic compression of stress fibers

American Journal of Physiology. Cell Physiology
Kazuaki NagayamaTakeo Matsumoto

Abstract

Actin stress fibers (SFs) of cells cultured on cyclically stretched substrate tend to reorient in the direction in which a normal strain of substrate becomes zero. However, little is known about the mechanism of this reorientation. Here we investigated the effects of cyclic stretch waveform on SF reorientation in osteoblastic cells. Cells adhering to silicone membranes were subjected to cyclic uniaxial stretch, having one of the following waveforms with an amplitude of 8% for 24 h: triangular, trapezoid, bottom hold, or peak hold. SF reorientation of these cells was then analyzed. No preferential orientation was observed for the triangular and the peak-hold waveforms, whereas SFs aligned mostly in the direction with zero normal strain (~55°) with other waveforms, especially the trapezoid waveform, which had a hold time both at loaded and unloaded states. Viscoelastic properties of SFs were estimated in a quasi-in situ stress relaxation test using intact and SF-disrupted cells that maintained their shape on the substrate. The dynamics of tension F(SFs) acting on SFs during cyclic stretching were simulated using these properties. The simulation demonstrated that F(SFs) decreased gradually during cyclic stretching and exhibited a ...Continue Reading

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Citations

Jun 16, 2012·American Journal of Physiology. Cell Physiology·Ramaswamy KrishnanDimitrije Stamenovic
Aug 16, 2013·Journal of Biomedical Materials Research. Part a·Matthew D MolesAlastair Campbell Ritchie
May 31, 2014·Nature Communications·Ariel LivneBenjamin Geiger
Aug 17, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Laura BernardiAldo Ferrari
Sep 12, 2019·Biomechanics and Modeling in Mechanobiology·Kazuaki Nagayama, Tomohiro Fukuei
Aug 11, 2020·Biomechanics and Modeling in Mechanobiology·Takumi SaitoShinji Deguchi
Jul 19, 2019·The Journal of Cell Biology·Abhishek KumarMartin A Schwartz

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