Strains of the Harmful Cyanobacterium Microcystis aeruginosa Differ in Gene Expression and Activity of Inorganic Carbon Uptake Systems at Elevated CO2 Levels

Applied and Environmental Microbiology
Giovanni SandriniJef Huisman

Abstract

Cyanobacteria are generally assumed to be effective competitors at low CO2 levels because of their efficient CO2-concentrating mechanism (CCM), and yet how bloom-forming cyanobacteria respond to rising CO2 concentrations is less clear. Here, we investigate changes in CCM gene expression at ambient CO2 (400 ppm) and elevated CO2 (1,100 ppm) in six strains of the harmful cyanobacterium Microcystis. All strains downregulated cmpA encoding the high-affinity bicarbonate uptake system BCT1, whereas both the low- and high-affinity CO2 uptake genes were expressed constitutively. Four strains downregulated the bicarbonate uptake genes bicA and/or sbtA, whereas two strains showed constitutive expression of the bicA-sbtA operon. In one of the latter strains, a transposon insert in bicA caused low bicA and sbtA transcript levels, which made this strain solely dependent on BCT1 for bicarbonate uptake. Activity measurements of the inorganic carbon (Ci) uptake systems confirmed the CCM gene expression results. Interestingly, genes encoding the RuBisCO enzyme, structural carboxysome components, and carbonic anhydrases were not regulated. Hence, Microcystis mainly regulates the initial uptake of inorganic carbon, which might be an effective str...Continue Reading

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Citations

Aug 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Giovanni SandriniJef Huisman
Sep 16, 2017·Journal of Experimental Botany·John Beardall, John A Raven
Aug 3, 2017·Critical Reviews in Biotechnology·Yimin ChenSeetharaman Vaidyanathan
Sep 26, 2019·Frontiers in Microbiology·Emily J DavenportRobert Michael L McKay
Sep 9, 2020·Bioelectrochemistry·Rita Vieira LemosStefano Freguia
Jan 12, 2017·Harmful Algae·Petra M VisserJef Huisman
Jun 1, 2021·Environmental Microbiology·Gregory J DickVincent J Denef

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