Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis

The Journal of Infectious Diseases
T J HellyerK D Eisenach

Abstract

Specific amplification of Mycobacterium tuberculosis DNA was investigated as an alternative to conventional microbiologic follow-up in 31 cases of smear- and culture-positive pulmonary tuberculosis. Strand displacement amplification (SDA) and the polymerase chain reaction (PCR) were applied to 438 sequential sputum specimens: 67 (15%) were positive by culture, 248 (57%) by SDA, and 231 (53%) by PCR (chi2=3.94, P=.05). Of 200 specimens collected >180 days after treatment started, none yielded positive cultures, while 50 (25%), representing 16 patients, were positive by both DNA assays. A weak correlation was demonstrated between DNA persistence in sputum and duration of culture positivity (r=0.45, P=.01), although no correlation was found with the radiographic extent of disease. The inability to distinguish live and dead organisms precludes DNA amplification from use in therapeutic monitoring. For this purpose, quantitative RNA assays are needed if such techniques are to supplant conventional microbiology.

Citations

Jul 20, 2002·Current Infectious Disease Reports·Hong-Zhou LuYi-Wei Tang
Sep 1, 1999·The Journal of Infection·G E Pfyffer
Jan 18, 2003·Journal of Microbiological Methods·Henriette StavriR J Doyle
Jan 17, 2014·The Lancet. Respiratory Medicine·Sven O FriedrichUNKNOWN Pan African Consortium for the Evaluation of Anti-tuberculosis Antibiotics (PanACEA)
Feb 15, 2002·Respirology : Official Journal of the Asian Pacific Society of Respirology·N ChierakulS N Pattanakitsakul
Oct 5, 2007·The New England Journal of Medicine·Henry M FederL Zemel
Nov 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Seigo KitadaKazuo Kobayashi
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sonia H MontenegroRichard A Oberhelman
May 26, 2006·Antimicrobial Agents and Chemotherapy·Steve AellenPhilippe Moreillon
Jul 25, 2000·Clinical Microbiology Reviews·M J HomerD H Persing
Jun 7, 2003·Journal of Clinical Microbiology·S Honoré-BouaklineJ L Herrmann
Jul 10, 2004·Journal of Clinical Microbiology·Isik Somuncu JohansenVibeke Østergaard Thomsen
Jan 7, 2005·Journal of Clinical Microbiology·Clarisse RoveryDidier Raoult
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·R A CohenK Kaul
Jul 3, 1999·American Journal of Respiratory and Critical Care Medicine·L E DesjardinK D Eisenach
Dec 15, 2007·American Journal of Respiratory and Critical Care Medicine·Seigo KitadaUNKNOWN MAC Serodiagnosis Study Group
Jul 20, 2010·Diagnostic Microbiology and Infectious Disease·Stefan Panaiotov, Massimo Amicosante
May 17, 2005·Diagnostic Microbiology and Infectious Disease·Douglas F MooreLydia T Mikhail
Mar 6, 2004·Expert Review of Molecular Diagnostics·Tobin J Hellyer, James G Nadeau
Jun 30, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C M ShoenM H Cynamon
Oct 19, 1999·Journal of Clinical Microbiology·V O ThomsenH J Burkardt
Mar 1, 1997·Journal of Clinical Microbiology·S H GillespieL E Newport
Apr 1, 1997·Journal of Clinical Microbiology·B L StoneM L Wilson
Jan 16, 1999·Journal of Clinical Microbiology·T J HellyerK D Eisenach
Oct 1, 1996·Journal of Clinical Microbiology·L E DesjardinK D Eisenach
Nov 1, 1996·Journal of Clinical Microbiology·T J HellyerK D Eisenach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.