Strand-specific miR-28-3p and miR-28-5p have differential effects on nasopharyngeal cancer cells proliferation, apoptosis, migration and invasion

Cancer Cell International
Yan LvGeping Wu

Abstract

MicroRNAs (miRNAs) play crucial roles in varieties of cancers, particularly in tumorigenesis, progression, and migration. Dysregulation of miR-28 was reported to occur in various types of human malignancies. In humans, two different mature miRNA sequences are excised from opposite arms of the stem-loop pre-miR-28, hsa-miR-28-3p and hsamiR-28-5p. However, the expression and distinct role of miR-28-3p and miR-28-5p in nasopharyngeal carcinoma (NPC) remain undetermined. The expressions of miR-28-3p/-5p in human NPC tissues were tested by quantitative real-time PCR. miR-28-3p/-5p were overexpressed by mimics and silenced by inhibitors. The roles of miR-28-3p/-5p in NPC development were studied using cultured HONE-1 cells. The mRNA expression levels of miR-28-3p and -5p were significantly decreased in NPC tissues in comparison with adjacent normal tissues. Overexpression of miR-28-5p suppressed NPC cell proliferation and induced cell cycle arrest and apoptosis, while miR-28-3p promoted NPC cell migration and invasion. The miRNAs effected on different signal pathways: miR-28-5p altered expression of cyclin D1 and influenced the PI3K/AKT signaling pathway. In contrast, miR-28-3p downregulated Nm23-H1 and accelerated the process of EMT...Continue Reading

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Citations

Mar 3, 2020·Journal of Toxicology and Environmental Health. Part a·Kihun KimYun Hak Kim
Jul 10, 2020·Acta Biochimica Et Biophysica Sinica·Liang ZhangZhe Sun
Sep 9, 2020·Cancer Biotherapy & Radiopharmaceuticals·Yuntong GuoHe Huang
Sep 26, 2020·Journal of Receptor and Signal Transduction Research·Kai SangLei Yu

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Methods Mentioned

BETA
transfection
antisense oligonucleotides
PCR
flow cytometry
flow

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