Strategies for Creating Structure-Switching Aptamers

ACS Sensors
Trevor A FeaginHyongsok Tom Soh

Abstract

Aptamer biosensor that can switch its structure upon target binding offers a powerful strategy for molecular detection. However, the process of converting an aptamer into a "structure-switching" biosensor is challenging and often relies on trial-and-error without established design principles. In this Sensor Issues, we examine a variety of design approaches for incorporating structure-switching functionality into existing aptamers, and provide thermodynamic analyses to highlight the variables that most strongly influence their performance. Finally, we also describe emerging efforts for incorporating the structure-switching functionality directly into the aptamer selection process.

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Citations

Feb 13, 2019·Nanomaterials·Zhining SunMingxu You
Nov 9, 2019·Nature Communications·Brandon D WilsonH Tom Soh
Feb 2, 2019·Chemical Society Reviews·Jeffrey D MunzarDavid Juncker
Nov 15, 2019·Scientific Reports·Mohammed Dwidar, Yohei Yokobayashi
Nov 10, 2020·Advanced Materials·Alexandra E RangelH Tom Soh
Apr 13, 2020·Analytica Chimica Acta·Emma DuffyAren E Gerdon
Mar 27, 2021·Molecular Psychiatry·Nako NakatsukaKrishna C Vadodaria
Mar 25, 2019·Methods : a Companion to Methods in Enzymology·Alexis AutourMichael Ryckelynck
May 14, 2021·Trends in Biotechnology·Jiahui Zhang, Khalid Salaita
Jun 11, 2021·Analytical and Bioanalytical Chemistry·Annelies DillenJeroen Lammertyn
Aug 4, 2021·Nature Communications·Qiu-Long ZhangLiang Xu
Aug 14, 2020·Journal of Agricultural and Food Chemistry·Yong ZhangRuijie Deng
Jul 8, 2019·ACS Biomaterials Science & Engineering·Jacob ShlafermanAren E Gerdon
Oct 16, 2019·Analytical Chemistry·Hualong GaoXinhui Lou
Sep 4, 2020·Biophysical Journal·Zachary R ChurcherPhilip E Johnson

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