Strategies for Determining the Bioactive Ingredients of Honey-processed Astragalus by Serum Pharmacochemistry integrated with Multivariate Statistical Analysis

Journal of Separation Science
Jing HuangHongyuan Chen

Abstract

Honey-processed Astragalus is a widely used traditional Chinese medicine which has a better effect on reinforcing "Qi" (vital energy) than the raw one. A comparative study of metabolites analysis between them in rat serum for finding the bioactive ingredients was carried out using serum pharmacochemistry and multivariate statistical analysis. The blood collection methods and time were optimized first. Then the prototypes and metabolites of serum samples after oral administration were investigated by ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry integrated with principal component analysis and orthogonal partial least squares discriminant analysis. The contents of metabolites were also analyzed to evaluate the metabolic profile differences. As a result, 9 prototypes and 36 metabolites were identified. Only 2 prototypes and 15 metabolites were different between raw and honey-processed Astragalus. Their biotransformation reactions contained phase I which consisted of the process of oxidation, demethylation and hydrolysis and phase II which included the glucuronide conjugation or sulfate conjugation. Most of the detected metabolites were transformed fro...Continue Reading

References

Mar 2, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Feng XuShaoqing Cai
Jul 19, 2008·Journal of Asian Natural Products Research·Yue CongXian Li
Jun 28, 2011·Journal of Pharmaceutical and Biomedical Analysis·Luying ChenChenggang Huang
Feb 5, 2013·Pharmaceutical Biology·Xiao-Yan HeChun-Jie Wu
May 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ya-Zhou ZhangHu-Biao Chen
Jun 13, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei ZhangJin-Ao Duan
Oct 17, 2015·Food Chemistry·Jing ZhaoWei Cao

❮ Previous
Next ❯

Citations

Jun 6, 2021·Fitoterapia·Faqin WangHuiqin Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.