Abstract
High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing. A novel wild-type Clostridium sp. strain NJP7 was isolated and characterized in this study, which was capable of fermenting monosaccharides, e.g., glucose into butanol via a fermentative acetone-isopropanol-butanol pathway. With enhancement of buffering capacity and alcohol dehydrogenase activities, butanol and isopropanol titer by Clostridium sp. strain NJP7 was improved to 12.21 and 1.92 g/L, respectively, and solvent productivity could be enhanced to 0.44 g/L/h. Furthermore, with in situ extraction with biodiesel, the amount of butanol and isopropanol was finally improved to 25.58 and 5.25 g/L in the fed-batch mode. Meanwhile,...Continue Reading
References
Jan 1, 1997·Advances in Applied Microbiology·P Bajpai
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Jan 30, 1999·Applied and Environmental Microbiology·C K Chen, H P Blaschek
Jul 24, 2001·Journal of Bacteriology·J NöllingD R Smith
Sep 11, 2001·Applied Microbiology and Biotechnology·Q K BegG S Hoondal
Jan 10, 2002·Journal of Industrial Microbiology & Biotechnology·N Qureshi, H P Blaschek
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Mar 1, 1983·Applied and Environmental Microbiology·H A GeorgeJ S Chen
Dec 1, 1984·Applied and Environmental Microbiology·R A HoltJ G Morris
Apr 1, 1987·Applied and Environmental Microbiology·S F HiuJ S Chen
Oct 16, 2007·Applied and Environmental Microbiology·T HanaiJ C Liao
Aug 30, 2008·Biotechnology and Bioengineering·Sang Yup LeeKwang S Jung
Mar 8, 2011·Applied and Environmental Microbiology·Wendy HigashideJames C Liao
May 17, 2011·Applied Microbiology and Biotechnology·Shrikant A SurvaseTom Granström
Jul 19, 2011·Applied and Environmental Microbiology·Shunichi NakayamaAtsumi Nakazato
Nov 15, 2011·Biotechnology Journal·Yang GuSheng Yang
Jan 3, 2012·Applied and Environmental Microbiology·Joungmin LeeSang Yup Lee
Mar 22, 2012·BMC Genomics·Yi WangHans P Blaschek
Jan 31, 2013·World Journal of Microbiology & Biotechnology·Zhi Rui NgZhibin Liu
Dec 3, 2014·Biotechnology Advances·Yi-Heng Percival Zhang
Jul 15, 2015·Metabolic Engineering·Paul P LinJames C Liao
Sep 15, 2015·Metabolic Engineering·Xiaorui YangShang-Tian Yang
Oct 26, 2016·Biotechnology for Biofuels·Fengxue XinMin Jiang
Citations
Sep 16, 2017·Critical Reviews in Biotechnology·Fengxue XinMin Jiang
Feb 23, 2018·Biotechnology for Biofuels·Chongran SunYi-Rui Wu
Dec 12, 2018·Biotechnology for Biofuels·Banggui ChengJunli Ren
Sep 27, 2018·Biotechnology for Biofuels·Fengxue XinMin Jiang
Jun 9, 2018·Biotechnology for Biofuels·Zhongxue DaiFengxue Xin
May 8, 2018·Applied Microbiology and Biotechnology·Yujia JiangFengxue Xin
Feb 8, 2019·3 Biotech·Jiasheng LuFengxue Xin
Sep 12, 2020·Applied Microbiology and Biotechnology·Nils ThiemeVladimir Zverlov
Mar 23, 2018·Current Microbiology·Chaoyang ChenYi-Rui Wu
Oct 25, 2017·Current Microbiology·Yujia JiangFengxue Xin
Apr 14, 2019·Applied and Environmental Microbiology·Paula Dalcin MartinsMichael J Wilkins
Sep 19, 2020·Biotechnology and Bioengineering·Yujia JiangMin Jiang
Mar 14, 2020·Biotechnology for Biofuels·Shubo LiLiming Liu
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Dec 22, 2020·Trends in Biotechnology·Yonghao CuiKang Zhou
Oct 31, 2018·Journal of Agricultural and Food Chemistry·Banggui ChengJunli Ren
Jun 5, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Chih-Yung ChiuWen-Hung Chung