Strategies for improved isopropanol-butanol production by a Clostridium strain from glucose and hemicellulose through consolidated bioprocessing

Biotechnology for Biofuels
Fengxue XinMin Jiang

Abstract

High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing. A novel wild-type Clostridium sp. strain NJP7 was isolated and characterized in this study, which was capable of fermenting monosaccharides, e.g., glucose into butanol via a fermentative acetone-isopropanol-butanol pathway. With enhancement of buffering capacity and alcohol dehydrogenase activities, butanol and isopropanol titer by Clostridium sp. strain NJP7 was improved to 12.21 and 1.92 g/L, respectively, and solvent productivity could be enhanced to 0.44 g/L/h. Furthermore, with in situ extraction with biodiesel, the amount of butanol and isopropanol was finally improved to 25.58 and 5.25 g/L in the fed-batch mode. Meanwhile,...Continue Reading

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Citations

Dec 12, 2018·Biotechnology for Biofuels·Banggui ChengJunli Ren
Sep 27, 2018·Biotechnology for Biofuels·Fengxue XinMin Jiang
Jun 9, 2018·Biotechnology for Biofuels·Zhongxue DaiFengxue Xin
May 8, 2018·Applied Microbiology and Biotechnology·Yujia JiangFengxue Xin
Sep 12, 2020·Applied Microbiology and Biotechnology·Nils ThiemeVladimir Zverlov
Apr 14, 2019·Applied and Environmental Microbiology·Paula Dalcin MartinsMichael J Wilkins
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Dec 22, 2020·Trends in Biotechnology·Yonghao CuiKang Zhou
Jun 5, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Chih-Yung ChiuWen-Hung Chung

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Datasets Mentioned

BETA
KX378860

Methods Mentioned

BETA
PCR
protein assay

Software Mentioned

MEGA
Basic Local Alignment Search Tool ( BLAST )
CLUSTAL X

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