Strategies for selecting recombinant CHO cell lines for cGMP manufacturing: improving the efficiency of cell line generation

Biotechnology Progress
Alison J PorterAlan J Dickson

Abstract

Transfectants with a wide range of cellular phenotypes are obtained during the process of cell line generation. For the successful manufacture of a therapeutic protein, a means is required to identify a cell line with desirable growth and productivity characteristics from this phenotypically wide-ranging transfectant population. This identification process is on the critical path for first-in-human studies. We have stringently examined a typical selection strategy used to isolate cell lines suitable for cGMP manufacturing. One-hundred and seventy-five transfectants were evaluated as they progressed through the different assessment stages of the selection strategy. High producing cell lines, suitable for cGMP manufacturing, were identified. However, our analyses showed that the frequency of isolation of the highest producing cell lines was low and that ranking positions were not consistent between each assessment stage, suggesting that there is potential to improve upon the strategy. Attempts to increase the frequency of isolation of the 10 highest producing cell lines, by in silico analysis of alternative selection strategies, were unsuccessful. We identified alternative strategies with similar predictive capabilities to the ty...Continue Reading

References

Feb 15, 2001·Biochemical Engineering Journal·C -M. Liu, L -N. Hong
Jan 17, 2003·Biotechnology and Bioengineering·Louise M BarnesAlan J Dickson
Mar 3, 2004·Biotechnology and Bioengineering·Jing-Xiu BiMohamed Al-Rubeai
Dec 25, 2004·Biotechnology and Bioengineering·Natalie MullerFlorian M Wurm
Feb 8, 2006·Trends in Biotechnology·Ted H J Kwaks, Arie P Otte
Oct 20, 2006·PLoS Biology·Arjun RajSanjay Tyagi
Nov 23, 2006·Biotechnology and Bioengineering·Scott H StansfieldDavid C James
Dec 19, 2006·Molecular Biotechnology·Stephan KalwyRobert Young
Jul 31, 2007·Trends in Biotechnology·Susan M Browne, Mohamed Al-Rubeai
Mar 24, 2009·Cytotechnology·Kristina LindgrenUlrica Skoging-Nyberg

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Citations

Apr 29, 2015·Biotechnology Progress·Peter M O'CallaghanDulce Aldana
Jul 23, 2015·Biotechnology Journal·Christopher A SellickAlan J Dickson
Aug 7, 2013·Biotechnology Advances·Vaibhav JadhavNicole Borth
May 15, 2015·Biotechnology Letters·Huong LeWei-Shou Hu
Jun 9, 2016·Biotechnology Journal·Joseph J PriolaMichael J Betenbaugh
Jun 18, 2016·Biotechnology Journal·Joseph K ChengHal S Alper
Oct 10, 2018·Biotechnology Progress·Benjamin WangPamela Hawley-Nelson
Mar 31, 2011·Biotechnology Progress·Rachel LegmannA Peter Russo
Jul 27, 2012·Biotechnology and Bioengineering·Sarah L DaviesDavid C James
Sep 30, 2021·Biotechnology and Bioengineering·Hirra HussainChristopher M Smales

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