Strategies to Encapsulate the Staphylococcus aureus Bacteriophage phiIPLA-RODI

Viruses
Eva González-MenéndezPilar García

Abstract

The antimicrobial properties of bacteriophages make them suitable food biopreservatives. However, such applications require the development of strategies that ensure stability of the phage particles during food processing. In this study, we assess the protective effect of encapsulation of the Staphylococcus aureus bacteriophage phiIPLA-RODI in three kinds of nanovesicles (niosomes, liposomes, and transfersomes). All these systems allowed the successful encapsulation of phage phiIPLA-RODI with an efficiency ranged between 62% and 98%, regardless of the concentration of components (like phospholipids and surfactants) used for vesicle formation. Only niosomes containing 30 mg/mL of surfactants exhibited a slightly lower percentage of encapsulation. Regarding particle size distribution, the values determined for niosomes, liposomes, and transfersomes were 0.82 ± 0.09 µm, 1.66 ± 0.21 µm, and 0.55 ± 0.06 µm, respectively. Importantly, bacteriophage infectivity was maintained during storage for 6 months at 4 °C for all three types of nanovesicles, with the exception of liposomes containing a low concentration of components. In addition, we observed that niosomes partially protected the phage particles from low pH. Thus, while free phi...Continue Reading

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Citations

Aug 14, 2019·Viruses·Sílvio B Santos, Joana Azeredo
Aug 25, 2019·Antibiotics·Lucía FernándezAna Rodríguez
Oct 20, 2020·Frontiers in Microbiology·Stijn Gerard RotmanThomas Fintan Moriarty
Jun 18, 2021·Frontiers in Cellular and Infection Microbiology·Israa M Abd-AllahNadia A Hassouna
Jul 17, 2021·Advanced Drug Delivery Reviews·Wei YanSharon S Y Leung

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Methods Mentioned

BETA
Dynamic Light Scattering
phage titration

Software Mentioned

PhageGuard Listex
PhageGuard
SPSS Statistics

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