PMID: 11604172Oct 18, 2001Paper

Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression

Journal of Biotechnology
A V CarvalhalM J T Carrondo

Abstract

Activation of the constitutively expressed interferon-regulatory-factor-1/estrogen receptor fusion protein (IRF-1-hER) in BHK cells was accomplished through the addition of estradiol to the culture medium, which enabled IRF-1 to gain its transcriptional activator function and inhibit cell growth. With the addition of 100 nM estradiol at the beginning of the exponential phase of a cell suspension culture, IRF-1 activation led to a rapid cell growth inhibition but also to a significant decrease in cell viability. To apply this concept in industry, a reduction of the time span of estradiol exposure is required. Cycles of estradiol addition and removal were performed in 2-l stirred tank bioreactors operated under perfusion, where an initial step addition of 100 nM estradiol was performed, followed, after 48-72 h, by a slow dilution with estradiol-free fresh medium (perfusion rate varying between 0.7 and 1.4 per day). Cell growth inhibition was successfully achieved for three consecutive cycles. Diluting the estradiol by perfusing medium without estradiol to concentrations lower than 10 nM led to cell growth and viability recovery independently of the perfusion rate used. These observations permitted the definition of operational st...Continue Reading

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Citations

Nov 13, 2008·Cytotechnology·Darrin KuystermansMohamed Al-Rubeai
Mar 3, 2004·Biotechnology and Bioengineering·Jing-Xiu BiMohamed Al-Rubeai

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