PMID: 8938977Jan 1, 1996Paper

Strategy for identifying the gene encoding the DNA polymerase of molluscum contagiosum virus type 1

Virus Genes
K C Sonntag, G Darai

Abstract

Molluscum contagiosum virus (MCV) is a member of the family Poxviridae and pathogenic to humans. MCV causes benign epidermal tumors mainly in children and young adults and is a common pathogen in immunecompromised individuals. The viral DNA polymerase is the essential enzyme involved in the replication of the genome of DNA viruses. The identification and characterization of the gene encoding the DNA polymerase of molluscum contagiosum virus type 1 (MCV-1) was carried out by PCR technology and nucleotide sequence analysis. Computer-aided analysis of known amino acid sequences of DNA polymerases from two members of the poxvirus family revealed a high amino acid sequence homology of about 49.7% as detected between the DNA polymerases of vaccinia virus (genus Orthopoxvirus) and fowlpoxvirus (genus Avipoxvirus). Specific oligonucleotide primers were designed and synthesized according to the distinct conserved regions of amino acid sequences of the DNA polymerases in which the codon usage of the MCV-1 genome was considered. Using this technology a 228 bp DNA fragment was amplified and used as hybridization probe for identifying the corresponding gene of the MCV-1 genome. It was found that the PCR product was able to hybridize to the ...Continue Reading

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Citations

Jul 20, 2010·Interdisciplinary Sciences, Computational Life Sciences·Rui ZhuDennis R Salahub
Dec 7, 2014·Journal de gynécologie, obstétrique et biologie de la reproduction·S WarembourgV Letouzey
Feb 22, 2014·PloS One·Subuhi SherwaniJoachim Jakob Bugert

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