Strategy for increased efficiency of transfection in human cell lines using radio frequency electroporation

Preparative Biochemistry & Biotechnology
P B ZaldE S Robertso

Abstract

Traditional electroporation devices use direct current electric fields to stimulate the uptake of oligonucleotides, plasmids, short peptides, and proteins into a variety of cell types. A variation of this widely used technique is now available which relies on radio frequency (RF) electrical pulses. This oscillating type of electrical field reportedly elicits greater uptake of plasmid DNA across the plasma membrane. We evaluated a protocol for RF electroporation of the a human embryonic kidney cell line and a Burkitt's lymphoma (BL) cell line for effeciency of transfection by RF electroporation. The plasmid EGFP, which codes for the widely used fusion protein, enhanced green fluorescent protein (EGFP), was used as a reporter of plasmid uptake after transfections. Transfection efficiency consistently increased approximately 30% from that typically obtained with conventional DC type electroporation and was accompanied by greater survivability of cells. Additionally, in some instances, percent transfection efficiency increased to over 70%. Thus, RF electroporation represents an improved methodology for transfection of human cell lines. Moreover, the RF protocol is simple to incorporate in laboratories already utilizing conventional...Continue Reading

References

Jan 17, 1991·Biochimica Et Biophysica Acta·Q A Zheng, D C Chang
Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jun 8, 1993·Biochimica Et Biophysica Acta·S Orlowski, L M Mir
Nov 1, 1995·Trends in Biochemical Sciences·A B CubittR Y Tsien
Mar 6, 1999·Biochemical and Biophysical Research Communications·R A MeldrumS Richardson
Sep 5, 2008·Current Protocols in Cytometry·Peter S Rabinovitch, J Paul Robinson

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Citations

Dec 8, 2005·Applied and Environmental Microbiology·Michael V TyurinLee R Lynd
May 17, 2005·Nitric Oxide : Biology and Chemistry·Karsten HemmrichVictoria Kolb-Bachofen

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Methods Mentioned

BETA
transfection
fluorescence
fluorescence microscopy
transfections

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