Stratifying risk of recurrence in stage II colorectal cancer using deregulated stromal and epithelial microRNAs

Oncotarget
Marc D BullockAlex H Mirnezami

Abstract

MicroRNAs (miRNAs) enable colonic epithelial cells to acquire malignant characteristics and metastatic capabilities. Recently, cancer relevant miRNAs deregulated during disease progression have also been identified in tumor-associated stroma.By combining laser-microdissection (LMD) with high-throughput screening and high-sensitivity quantitation techniques, miRNA expression in colorectal cancer (CRC) specimens and paired normal colonic tissue was independently characterized in stromal and epithelial tissue compartments. Notably, deregulation of the key oncogene miR-21 was identified exclusively as a stromal phenomenon and miR-106a, an epithelial phenomenon in the malignant state.MiRNAs identified in this study successfully distinguished CRC from normal tissue and metastatic from non-metastatic tumor specimens. Furthermore, in a separate cohort of 50 consecutive patients with CRC, stromal miR-21 and miR-556 and epithelial miR-106a expression predicted short disease free survival (DFS) and overall survival (OS) in stage II disease: miR-21 (DFS: HR = 2.68, p = 0.015; OS: HR = 2.47, p = 0.029); miR-556 (DFS: HR = 2.60, p = 0.018); miR-106a (DFS: HR = 2.91, p = 0.008; OS: HR = 2.25, p = 0.049); combined (All High vs. All Low. DFS: H...Continue Reading

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Citations

Jun 9, 2016·New Horizons in Translational Medicine·R BhomeA H Mirnezami
Feb 6, 2020·Molecular Diagnosis & Therapy·Shanthi SabarimuruganRama Jayaraj
Jun 23, 2016·Oncotarget·Lise M LindahlNiels Odum
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Nov 19, 2019·Journal of Oncology·Valentin VautrotJessica Gobbo
Mar 22, 2018·Oncotarget·Steven A SignsEmina H Huang
Jan 24, 2021·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·Rahul BhomeZaed Hamady
May 1, 2021·Cancers·Inge van den BergSaskia M Wilting
Jul 3, 2021·Cancers·Paulína Pidíková, Iveta Herichová

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Methods Mentioned

BETA
PCR
laser capture microdissection
Assay

Software Mentioned

GenePixPro
MIAME
HTqPCR
miRCURY
R Bioconductor

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