Streamlined Construction of the Cyanobacterial CO2-Fixing Organelle via Protein Domain Fusions for Use in Plant Synthetic Biology

The Plant Cell
C Raul Gonzalez-EsquerCheryl A Kerfeld

Abstract

Bacterial microcompartments (BMCs) are self-assembling organelles that sequester segments of biochemical pathways within a protein shell. Given their functional diversity, BMCs constitute a rich source of metabolic modules for applications in synthetic biology. The carboxysome, the cyanobacterial BMC for CO(2) fixation, has attracted significant attention as a target for installation into chloroplasts and serves as the foundation for introducing other types of BMCs into plants. Carboxysome assembly involves a series of protein-protein interactions among at least six gene products to form a metabolic core, around which the shell assembles. This complexity creates significant challenges for the transfer, regulation, and assembly of carboxysomes, or any of the myriad of functionally distinct BMCs, into heterologous systems. To overcome this bottleneck, we constructed a chimeric protein in the cyanobacterium Synechococcus elongatus that structurally and functionally replaces four gene products required for carboxysome formation. The protein was designed based on protein domain interactions in the carboxysome core. The resulting streamlined carboxysomes support photosynthesis. This strategy obviates the need to regulate multiple gen...Continue Reading

Citations

Feb 18, 2016·Applied Microbiology and Biotechnology·Xinyan GaoWeiwen Zhang
Jan 9, 2016·Microbial Cell Factories·Jie ZhouYin Li
Mar 22, 2016·Current Opinion in Plant Biology·Benedict M LongG Dean Price
Feb 13, 2016·The Plant Journal : for Cell and Molecular Biology·Maureen R HansonMartin A J Parry
Mar 19, 2016·The Plant Journal : for Cell and Molecular Biology·C Raul Gonzalez-EsquerCheryl A Kerfeld
Jul 12, 2016·Current Opinion in Chemical Biology·Tobias J Erb, Jan Zarzycki
Jul 28, 2016·Applied Microbiology and Biotechnology·Maureen B QuinClaudia Schmidt-Dannert
Sep 27, 2016·Nature Reviews. Microbiology·Nico J ClaassensJohn van der Oost
Mar 10, 2016·PLoS Biology·Onur ErbilginCheryl A Kerfeld
Oct 14, 2016·PloS One·Getachew Tesfaye BeyeneTone Tønjum
Jan 28, 2017·Current Opinion in Biotechnology·Tobias W Giessen, Pamela A Silver
Mar 6, 2018·Nature Reviews. Microbiology·Cheryl A KerfeldMarkus Sutter
Dec 15, 2015·American Journal of Botany·Cheryl A Kerfeld
Aug 16, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cheryl A Kerfeld
Apr 1, 2020·Metabolites·Xueqin LvLong Liu
Apr 27, 2017·Journal of Experimental Botany·Benjamin D RaeAlistair J McCormick
Jun 21, 2018·Frontiers in Plant Science·Yi FangLu-Ning Liu
May 31, 2018·Scientific Reports·Christopher M JakobsonNiall M Mangan
Aug 22, 2017·Frontiers in Microbiology·Eric J YoungDaniel C Ducat
Sep 22, 2017·FEMS Microbiology Letters·Aiko TurmoCheryl A Kerfeld
Sep 5, 2018·Nature Communications·Benedict M LongG Dean Price
Jun 20, 2020·Current Opinion in Biotechnology·Cheryl A Kerfeld, Markus Sutter
Apr 16, 2020·Biotechnology Advances·Aubrey M Demchuk, Trushar R Patel
Apr 10, 2016·Current Opinion in Plant Biology·Cheryl A Kerfeld, Matthew R Melnicki
Oct 17, 2017·Current Opinion in Biotechnology·Jefferson S Plegaria, Cheryl A Kerfeld
Apr 3, 2021·Current Opinion in Microbiology·Julia S Borden, David F Savage

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Metabolism

Baterial metabolism is how bacteria obtain the energy and nutrients they need to live and reproduce.The study of bacterial metabolism focuses on the chemical diversity of substrate oxidations and dissimilation reactions (reactions by which substrate molecules are broken down), which normally function in bacteria to generate energy. Also within the scope of bacterial metabolism is the study of the uptake and utilization of the inorganic or organic compounds required for growth and maintenance of a cellular steady state (assimilation reactions). Discover the latest research on bacterial metabolism here.