PMID: 11320507Apr 26, 2001Paper

Strength of mid-logarithmic and stationary phase Saccharopolyspora erythraea hyphae during a batch fermentation in defined nitrate-limited medium

Biotechnology and Bioengineering
Stuart Stocks, C R Thomas

Abstract

A method for measuring mechanical properties of Saccharopolyspora erythraea is reported with data from a batch fermentation. Briefly, hyphae were glued to the end of a tungsten filament mounted horizontally on a sensitive force transducer. Free ends of hyphae were trapped against a flat surface by a second probe. The force transducer and tungsten filament were then moved at a fixed rate, the hypha were strained, and the force resisting motion recorded. From these data the maximum force resisting motion is taken as the force at which breakage occurs. Hyphae from the mid-logarithmic phase of a simple batch fermentation on defined medium were found to have a breaking force of 890 +/- 160 nN (95% confidence), while stationary phase hyphae were weaker at 580 +/- 150 nN. Video recordings of the experiments allowed an approximation of breaking strain, which did not differ significantly between samples at 0.18 +/- 0.03. Electron microscopy was used to measure cell wall thickness, cell diameter, and hence cell wall cross-sectional area. The ultimate tensile strength was estimated to be 24 +/- 3 MPa with no difference between the two samples, the lower breaking force of the stationary phase hyphae being attributed to a thinner cell wall....Continue Reading

References

Nov 1, 1991·Applied Microbiology and Biotechnology·Z ZhangC R Thomas
Feb 1, 1989·Journal of Bacteriology·N H Mendelson, J J Thwaites
Nov 14, 1997·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·G Sunder-Plassmann, W H Hörl
Aug 5, 2000·Biochemical Society Transactions·C L ThomasG Schiavo
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT

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Citations

Dec 18, 2001·Biotechnology and Bioengineering·S M Stocks, C R Thomas
Jul 10, 2012·Journal of Biotechnology·Rainer KrullChristoph Wittmann
Aug 9, 2003·Physical Review Letters·Arezki Boudaoud
Oct 14, 2017·Scientific Reports·M R IslamR C Picu
Dec 18, 2020·Biophysical Journal·Amar VelicPrasad K D V Yarlagadda
Mar 22, 2019·Journal of Colloid and Interface Science·Simon CollettPaul A Ramsland
Jun 21, 2021·Colloids and Surfaces. B, Biointerfaces·Qianqian CuiDan Ge

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