Streptavidin-modified monodispersed magnetic poly(2-hydroxyethyl methacrylate) microspheres as solid support in DNA-based molecular protocols

Materials Science & Engineering. C, Materials for Biological Applications
Tagrid SalihDaniel Horák

Abstract

Molecular diagnostics may provide tailored and cost efficient treatment for infectious disease and cancer. Rolling circle amplification (RCA) of padlock probes guarantees high specificity to identify nucleic acid targets down to single nucleotide resolution in a multiplex fashion. This makes the assay suitable for molecular analysis of various diseases, and interesting to integrate into automated devices for point-of-care analysis. A critical prerequisite for many molecular assays is (i) target-specific isolation from complex clinical samples and (ii) removal of reagents, inhibitors and contaminants between reaction steps. Efficient solid supports are therefore essential to enable multi-step, multi-analyte protocols. Superparamagnetic micro- and nanoparticles, with large surface area and rapid liquid-phase kinetics, are attractive for multi-step protocols. Recently, streptavidin-modified magnetic monodispersed poly(2-hydroxyethyl methacrylate) (STV-mag.PHEMA) microspheres were developed by multiple swelling polymerization. They are easily separated by a magnet and exhibit low non-specific protein sorption. In this study, the performance and the binding efficiency of STV-mag.PHEMA was addressed by circle-to-circle amplification ...Continue Reading

References

Nov 4, 1998·Nucleic Acids Research·J BanérU Landegren
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik DahlMats Nilsson
Aug 25, 2006·Nature Methods·Jonas JarviusMats Nilsson
Feb 15, 2013·Lab on a Chip·Chen-Min ChangGwo-Bin Lee

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