PMID: 58958Jul 1, 1976

Streptococcal M protein extracted by nonionic detergent. I. Properties of the antiphagocytic and type-specific molecules

The Journal of Experimental Medicine
V A FischettiJ B Zabriskie

Abstract

Group A streptococcal M protein was extracted with nonionic detergent and subjected to a number of physical, chemical, and immunological tests. M protein thus extracted was composed of multiple protein bands, ranging from 35,000 down to 6,000 daltons, all having type-specific precipitating activity. The anti-phagocytic proteins, however, were limited to three molecular species having mol wt of 28,000, 31,000, and 35,000 daltons, and could be separated from those proteins that had only type specificity. Physical studies indicated that these proteins existed as individual asymmetrical molecules which were not aggregated. By radiolabeling M protein on living streptococci, it was determined that these protein bands were found on the streptococcal cell wall in this multiple form. Also, by pulse chase experiments supported by chemical and immunological data, evidence was obtained strongly suggesting that the smaller, type-specific molecules are used to assemble the larger, antiphagocytic proteins.

References

Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Nov 1, 1971·The Journal of Experimental Medicine·J RottaH Lackland
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·E N Fox, M K Wittner
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Aug 1, 1959·The Journal of Experimental Medicine·R C Lancefield

Citations

Mar 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·E N SemenovaM N Smirnova
Apr 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·S GatermannG Wanner
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E H BeacheyA H Kang
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B HoseinV A Fischetti
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B N ManjulaV A Fischetti
Nov 1, 1980·The Journal of Experimental Medicine·J G Spanier, P P Cleary
Jul 1, 1978·Antimicrobial Agents and Chemotherapy·J R Tagg, L W Wannamaker
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Jun 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·J Kolberg

Related Concepts

Antigenic Specificity
Antigens, Bacterial
Bacterial Proteins
Cell Wall
Phagocytosis
Bacteria, Flesh-Eating
Structure-Activity Relationship

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