PMID: 58958Jul 1, 1976

Streptococcal M protein extracted by nonionic detergent. I. Properties of the antiphagocytic and type-specific molecules

The Journal of Experimental Medicine
V A FischettiJ B Zabriskie


Group A streptococcal M protein was extracted with nonionic detergent and subjected to a number of physical, chemical, and immunological tests. M protein thus extracted was composed of multiple protein bands, ranging from 35,000 down to 6,000 daltons, all having type-specific precipitating activity. The anti-phagocytic proteins, however, were limited to three molecular species having mol wt of 28,000, 31,000, and 35,000 daltons, and could be separated from those proteins that had only type specificity. Physical studies indicated that these proteins existed as individual asymmetrical molecules which were not aggregated. By radiolabeling M protein on living streptococci, it was determined that these protein bands were found on the streptococcal cell wall in this multiple form. Also, by pulse chase experiments supported by chemical and immunological data, evidence was obtained strongly suggesting that the smaller, type-specific molecules are used to assemble the larger, antiphagocytic proteins.


Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Nov 1, 1971·The Journal of Experimental Medicine·J RottaH Lackland
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·E N Fox, M K Wittner
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Aug 1, 1959·The Journal of Experimental Medicine·R C Lancefield


Mar 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·E N SemenovaM N Smirnova
Apr 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·S GatermannG Wanner
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E H BeacheyA H Kang
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B HoseinV A Fischetti
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B N ManjulaV A Fischetti
Nov 1, 1980·The Journal of Experimental Medicine·J G Spanier, P P Cleary
Jul 1, 1978·Antimicrobial Agents and Chemotherapy·J R Tagg, L W Wannamaker
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Jun 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·J Kolberg

Related Concepts

Antigenic Specificity
Antigens, Bacterial
Bacterial Proteins
Cell Wall
Bacteria, Flesh-Eating
Structure-Activity Relationship

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.