PMID: 3760782Oct 1, 1986Paper

Streptococcal M protein size mutants occur at high frequency within a single strain

The Journal of Experimental Medicine
V A FischettiJ R Scott

Abstract

Streptococcal M protein, the antiphagocytic molecule on the surface of the organism, was previously found to exhibit extensive size heterogeneity between as well as within M serotypes. In this study, methods were devised to isolate M protein size mutants within a laboratory-grown culture. We were able to isolate three independent M protein deletion mutants and one additional mutant, which was derived from the first deletion mutant. We found that these deletion mutants occur at a frequency of approximately 1 in 2 X 10(3) CFUs in culture. Functional studies revealed that the deletion mutants were able to survive as well as the parental strain in human blood. They also had the determinants necessary to absorb opsonic antibodies as well as the parent. Pepsin digestion experiments localized the deletions within the N-terminal half of the M molecule, which is distal to the cell wall surface. This is the region of the molecule in which extensive sequence repeats are found. This is consistent with the suggestion that the size changes may be the result of homologous recombination between the repeat regions in the gene. These results support the idea that strains showing M protein size variation within successive clinical isolates from s...Continue Reading

References

Aug 1, 1977·The Journal of Infectious Diseases·V A FischettiJ B Zabriskie
Jun 1, 1985·The Journal of Experimental Medicine·V A FischettiJ R Scott
Mar 1, 1985·The Journal of Experimental Medicine·K F JonesV A Fischetti
Nov 1, 1969·The Journal of Experimental Medicine·J SwansonE C Gotschlich
Aug 19, 1983·Science·J R Scott, V A Fischetti
Jun 1, 1982·Cell·P Borst, G A Cross
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
Aug 1, 1959·The Journal of Experimental Medicine·R C LANCEFIELD

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Citations

Sep 1, 1988·European Journal of Epidemiology·B N Manjula
Oct 1, 1991·Epidemiology and Infection·M H GroschupJ F Timoney
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M G Caparon, J R Scott
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K F JonesV A Fischetti
Jun 1, 2006·Blood·Sara H BengtsonHeiko Herwald
Jul 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Stephen B BeresJames M Musser
Sep 1, 1988·Microbiological Reviews·H S Seifert, M So
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti
May 23, 1998·Journal of Clinical Microbiology·D L GardinerK S Sriprakash

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