Streptococcus agalactiae DNA polymerase I is an efficient reverse transcriptase

Biochimie
Fariborz Bahrami, Jean-Luc Jestin

Abstract

Genome annotations result generally from large sequence alignments by bioinformatics. Large scale biochemical data are more difficult to obtain. They derive for example from directed protein evolution experiments by selection. A previously reported directed enzyme evolution experiment by in vitro selection of Stoffel fragment variants of Taq DNA polymerase I was used here to predict the activities of Streptococcus agalactiae DNA polymerase I. The reverse transcriptase activity of S. agalactiae DNA polymerase I was measured and the kinetic parameters for this RNA-dependent DNA polymerase are given. RNA-templated DNA repair is suggested as a possible biological function for this biochemical activity.

References

Jun 1, 1994·PCR Methods and Applications·R C Cadwell, G F Joyce
May 2, 2003·Molecular Biotechnology·Heike StrobelJean-Luc Jestin
Sep 24, 2004·Journal of Biotechnology·Jean-Luc Jestin, Pierre Alexandre Kaminski
Aug 23, 2007·The Journal of Biological Chemistry·Harold R Lee, Kenneth A Johnson

❮ Previous
Next ❯

Citations

Oct 21, 2009·Journal of Molecular Evolution·J L Jestin, A Kempf
Apr 22, 2010·Molecular Biotechnology·Sophie Vichier-Guerre, Jean-Luc Jestin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.