Streptococcus mutans biofilm transient viscoelastic fluid behaviour during high-velocity microsprays

Journal of the Mechanical Behavior of Biomedical Materials
S FabbriP Stoodley

Abstract

Using high-speed imaging we assessed Streptococcus mutans biofilm-fluid interactions during exposure to a 60-ms microspray burst with a maximum exit velocity of 51m/s. S. mutans UA159 biofilms were grown for 72h on 10mm-length glass slides pre-conditioned with porcine gastric mucin. Biofilm stiffness was measured by performing uniaxial-compression tests. We developed an in-vitro interproximal model which allowed the parallel insertion of two biofilm-colonized slides separated by a distance of 1mm and enabled high-speed imaging of the removal process at the surface. S. mutans biofilms were exposed to either a water microspray or an air-only microburst. High-speed videos provided further insight into the mechanical behaviour of biofilms as complex liquids and into high-shear fluid-biofilm interaction. We documented biofilms extremely transient fluid behaviour when exposed to the high-velocity microsprays. The presence of time-dependent recoil and residual deformation confirmed the pivotal role of viscoelasticity in biofilm removal. The air-only microburst was effective enough to remove some of the biofilm but created a smaller clearance zone underlying the importance of water and the air-water interface of drops moving over the s...Continue Reading

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Citations

Aug 25, 2016·Journal of Dental Research·S FabbriP Stoodley
Sep 26, 2017·Nature Reviews. Microbiology·Hyun KooLuanne Hall-Stoodley
Aug 12, 2017·Environmental Microbiology·Stefania FabbriPaul Stoodley
Jun 28, 2018·Scientific Reports·Erin S GloagDaniel J Wozniak
Aug 31, 2019·Drugs·Claudia Vuotto, Gianfranco Donelli
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Kaiyu ZhangYang Wang
Aug 23, 2018·NPJ Biofilms and Microbiomes·Héloïse BoudarelMichel Grédiac
Jan 30, 2021·Frontiers in Microbiology·Josué JaraBelén Orgaz
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Harinash RaoPei Pei Chong
Jun 2, 2021·Clinical and Experimental Dental Research·Kristina BertlAndreas Stavropoulos
Oct 27, 2021·Advanced Drug Delivery Reviews·Sajid AsgharThierry F Vandamme

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