Streptococcus pneumoniae capsular polysaccharide is linked to peptidoglycan via a direct glycosidic bond to β-D-N -acetylglucosamine

Proceedings of the National Academy of Sciences of the United States of America
Thomas R Larson, J Yother

Abstract

For many bacteria, including those important in pathogenesis, expression of a surface-localized capsular polysaccharide (CPS) can be critical for survival in host environments. In Gram-positive bacteria, CPS linkage is to either the cytoplasmic membrane or the cell wall. Despite the frequent occurrence and essentiality of these polymers, the exact nature of the cell wall linkage has not been described in any bacterial species. Using the Streptococcus pneumoniae serotype 2 CPS, which is synthesized by the widespread Wzy mechanism, we found that linkage occurs via the reducing end glucose of CPS and the β-D-N-acetylglucosamine (GlcNAc) residues of peptidoglycan (PG). Hydrofluoric acid resistance, 31P-NMR, and 32P labeling demonstrated the lack of phosphodiester bonds, which typically occur in PG-polysaccharide linkages. Component sugar analysis of purified CPS-PG identified only CPS and PG sugars in the appropriate ratios, suggesting the absence of an oligosaccharide linker. Time of flight mass spectrometry confirmed a direct glycosidic linkage between CPS and PG and showed that a single CPS repeat unit can be transferred to PG. The linkage was acetolysis susceptible, indicative of a 1,6 glycosidic bond between CPS and the GlcNAc...Continue Reading

References

Aug 1, 1989·Infection and Immunity·A M BerryJ C Paton
Dec 22, 1967·Science·P W RobbinsA Wright
Apr 27, 2000·The Journal of Biological Chemistry·W Vollmer, A Tomasz
May 12, 2001·Infection and Immunity·A D Magee, J Yother
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
Mar 15, 2006·PLoS Genetics·Stephen D BentleyBrian G Spratt
May 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Judy K MoronaJames C Paton
Jun 8, 2006·Annual Review of Biochemistry·Chris Whitfield
Nov 8, 2006·Infection and Immunity·Aaron L NelsonJeffrey N Weiser
Oct 4, 2011·The EMBO Journal·Yoshikazu KawaiJeff Errington
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alice EberhardtWaldemar Vollmer
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lisa M WillisChris Whitfield
Apr 23, 2014·The Journal of Biological Chemistry·Yvonne Gar-Yun ChanDominique Missiakas
Jul 18, 2015·Microbiology Spectrum·Dominique H LimoliDaniel J Wozniak
Nov 5, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·R KulkarniJ M Soucie
Feb 17, 2017·Journal of Clinical Microbiology·K Aaron GenoMoon H Nahm

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Citations

Jul 12, 2018·Human Vaccines & Immunotherapeutics·Joon Young SongHo Seong Seo
Jun 12, 2019·Microbial Genomics·Andries J van TonderUNKNOWN The Global Pneumococcal Sequencing Consortium
Jul 19, 2020·Annual Review of Microbiology·Chris WhitfieldCaitlin Sande
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Chankyu LeeInhwan Lee
Sep 27, 2019·Frontiers in Microbiology·Moses B AyoolaBindu Nanduri
Mar 2, 2018·Frontiers in Microbiology·Mickaël DesvauxPascale Serror
Oct 30, 2018·Frontiers in Bioengineering and Biotechnology·Victor MoraisNorma Suárez
Nov 24, 2020·Phytopathology·Yuan Zeng, Amy O Charkowski
Dec 19, 2020·FEMS Microbiology Reviews·Tong SuLok-To Sham
Jan 23, 2021·International Journal of Molecular Sciences·Cordula StefanovićChristina Schäffer
Jan 12, 2021·Frontiers in Cellular and Infection Microbiology·Jennifer N LuckCarlos J Orihuela
Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz
Apr 13, 2019·Microbiology Spectrum·James C Paton, Claudia Trappetti
Nov 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Rei NakamotoLok-To Sham

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