Streptococcus pneumoniae early response genes to human lung epithelial cells.

BMC Research Notes
Xin-Ming SongA A Potter

Abstract

Streptococcus pneumoniae infection starts from colonization of the host respiratory tract where interaction with host respiratory tract epithelial cells occurs. To investigate pneumococcal genes that are involved in the early stage of interaction with host epithelial cells, transcriptional responses of an encapsulated pathogenic pneumococcal strain TIGR4 upon exposure to human lung epithelial cells A549 for 0.5 h and 1 h time periods were investigated by using TIGR (JCVI) microarray technology. Gene expression changes were validated by quantitative real-time PCR (qRT-PCR) analysis. We observed different transcriptional profiles at two incubation time periods in which most gene expressions were down-regulated at 0.5 h but up-regulated at 1 h. Many genes associated with ribonucleotide biosynthesis were down-regulated at both time points, whereas the genes associated with cell envelope, energy metabolism, transport and protein synthesis were mostly up-regulated at 1 h. Furthermore, these profiles were compared to the transcriptomes of a TIGR4-derived strain in response to human macrophages for the same time periods. We found one set of genes that exhibited similar expression changes upon exposure to both types of host cells, inclu...Continue Reading

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Citations

Jun 21, 2011·Omics : a Journal of Integrative Biology·Fabio BagnoliRino Rappuoli
Jul 7, 2009·Trends in Microbiology·Fiona M McCarthyShane C Burgess
Oct 27, 2015·Frontiers in Microbiology·Laura PaixãoAna R Neves
Mar 30, 2018·Journal of Aquatic Animal Health·Pradeep R DumpalaAttila Karsi
Jul 1, 2009·Infection and Immunity·Dane ParkerAlice Prince
Sep 22, 2021·MBio·Dhriti SinhaNicholas R De Lay

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Methods Mentioned

BETA
PCR

Software Mentioned

ArrayPipe
MIAME
Limma
Clone Manager Suite

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