Streptococcus pneumoniae recruits complement factor H through the amino terminus of CbpA

The Journal of Biological Chemistry
Ling LuJing-Ren Zhang

Abstract

Streptococcus pneumoniae, a human pathogen, is naturally capable of colonizing the upper airway and sometimes disseminating to remote tissue sites. Previous studies have shown that S. pneumoniae is able to evade complement-mediated innate immunity by recruiting complement factor H (FH), a complement alternative pathway inhibitor. Pneumococcal binding to FH has been attributed to choline-binding protein A (CbpA) of S. pneumoniae and its allelic variants, all of which are surface-exposed proteins. In this study, we sought to determine the molecular basis of the CbpA-FH binding interaction. Initial deletional analysis of the CbpA protein in strain D39 (capsular serotype 2) revealed that the N-terminal region of 89 amino acids in the mature CbpA protein is required for FH binding. Immunofluorescence microscopy analysis showed that this region of CbpA is also necessary for FH deposition to the surface of the intact pneumococci. Moreover, recombinant proteins representing the 104 amino acids of the N-terminal CbpA alone was sufficient for high affinity binding to FH (KD < 1 nm). The FH binding activity was finally localized to a 12-amino acid motif in the N-terminal CbpA by peptide mapping. Further kinetic analysis suggested that add...Continue Reading

References

Feb 6, 1990·Biochemistry·D Stigter, K A Dill
Jul 1, 1989·Infection and Immunity·A M BerryJames C Paton
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R D HorstmannV A Fischetti
Feb 1, 1987·The Journal of Experimental Medicine·Larry S McDanielDavid E Briles
Jul 1, 1986·The Journal of Antimicrobial Chemotherapy·R Austrian
Mar 1, 1984·Infection and Immunity·James C PatonA Ferrante
Dec 1, 1980·The Journal of Infectious Diseases·S W HoseaM M Frank
Jan 1, 1995·Infection and Immunity·V A FischettiVijay Pancholi
May 1, 1994·Journal of Bacteriology·J Yother, J M White
Sep 1, 1993·Molecular Microbiology·B J PearceH R Masure
Oct 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·E N Janoff, J B Rubins
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·David E BrilesM J Crain
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna M BlomB Dahlbäck
May 18, 2000·Microbes and Infection·R Rautemaa, Seppo Meri
Sep 1, 2000·The Journal of Biological Chemistry·Robert JanulczykLars Björck
Dec 13, 2000·The Journal of Biological Chemistry·Jens HellwageSeppo Meri
Apr 9, 2001·Infection and Immunity·A HåkanssonDavid E Briles
Apr 9, 2001·Infection and Immunity·Sandhya DaveLarry S McDaniel
May 12, 2001·Infection and Immunity·R B AlcantaraM J Gentry-Nielsen
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Oct 27, 2001·Applied and Environmental Microbiology·C K SungD A Morrison
Jan 29, 2002·The Journal of Biological Chemistry·Thomas AreschougGunnar Lindahl
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Feb 7, 2002·Journal of Microbiological Methods·Peter C Y LauDennis G Cvitkovitch
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeremy S BrownMarina Botto
Aug 14, 2003·Molecular Immunology·Hanna JarvaSeppo Meri
Sep 19, 2003·The Journal of Biological Chemistry·Ling LuJing-Ren Zhang
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Apr 23, 2004·Infection and Immunity·Francesco IannelliGianni Pozzi
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antti AlitaloSeppo Meri
May 28, 2004·Molecular Immunology·Santiago Rodríguez de CórdobaPilar Sánchez-Corral
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandhya DaveLarry S McDaniel

Citations

May 28, 2010·The Journal of Biological Chemistry·Vaibhav AgarwalSven Hammerschmidt
Jun 4, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Daniel P MillerRichard T Marconi
Apr 11, 2007·Infection and Immunity·Jingliang SuJing-Ren Zhang
Jan 16, 2007·Journal of Bacteriology·Zhuo Ma, Jing-Ren Zhang
Jul 14, 2010·BMC Microbiology·Cécile FroletAnne Marie Di Guilmi
Aug 2, 2008·PloS One·Huaiqing ChenJing-Ren Zhang
Sep 5, 2012·Biological Chemistry·Michal Potempa, Jan Potempa
Mar 28, 2008·Future Microbiology·Julie A Preston, David H Dockrell
Oct 7, 2014·Research in Microbiology·Xian-Fei ZengXiaoke Hao
Sep 26, 2015·Annual Review of Genetics·Florian DouamAlexander Ploss
May 9, 2014·Clinical and Vaccine Immunology : CVI·Cintia F M VadesilhoEliane N Miyaji
Jan 18, 2018·Nature Communications·Hong-Bo YangCong-Zhao Zhou
Jan 18, 2008·Journal of Molecular Recognition : JMR·Oksana Okhrimenko, Ilian Jelesarov
Nov 27, 2019·Journal of Bacteriology·Tiffany M ZarrellaGuangchun Bai
Sep 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vaibhav AgarwalAnna M Blom
Oct 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew P HerbertPaul N Barlow
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sven HammerschmidtPeter F Zipfel
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ling LuJing-Ren Zhang
Jul 22, 2010·International Journal of Immunopathology and Pharmacology·V BuhéP Youinou
Mar 1, 2014·Emerging Microbes & Infections·Xiaolei PanJing-Ren Zhang
Aug 31, 2018·The Journal of Antimicrobial Chemotherapy·Jordi CàmaraCarmen Ardanuy
Mar 8, 2017·Frontiers in Microbiology·Greiciely O AndreMichelle Darrieux

Related Concepts

CbpA protein, bacteria
Bacterial Proteins
Genes, Bacterial
Peptide Mapping
Proteins, Recombinant DNA
Streptococcus pneumoniae
Complement FACTOR H
Deletion Mutation
Homologous Sequences, Amino Acid
Tertiary Protein Structure

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