Streptococcus pneumoniae serotype epidemiology among PCV-10 vaccinated and unvaccinated children at Gertrude's Children's Hospital, Nairobi County: a cross-sectional study

F1000Research
Michael WalekhwaBeatrice Kabera

Abstract

Background:Streptococcus pneumoniae (SP n) serotype replacement and emergence of multidrug resistant SP n has exacerbated the need for continuous regional serotype surveillance. We investigated SP n serotypes circulating among children ≤5 years in Nairobi County. Methods:Streptococcus pneumoniae stocks stored at -70°C in brain heart infusion medium were thawed at room temperature for 30 minutes. In total, 10 µl of the stored SP n cells were suspended in 50 µl PBS and gently vortexed. About 10 µl of the suspended cells were added on to a glass slide and mixed with 10 µl pooled antisera. The glass slide was swirled gently while observing for any reaction. The process was repeated with individual groups under various antisera pools. Those serotypes that did not belong to any pool were typed directly until a positive agglutination reaction was observed. The cells/PBS/serotype-specific antisera mixture on the glass slide were covered with a coverslip and observed under a phase contrast microscope at ×100 objective lens with oil emulsion. Results: Out of the 206 subjects sampled, 20.39% (n=42) were found to be carriers of SP n. About 52% (n=22) of the SP n carriers had received the recommended dose of PCV-10, while 48% (n=20) of the ...Continue Reading

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