Streptococcus pyogenes Ser/Thr kinase-regulated cell wall hydrolase is a cell division plane-recognizing and chain-forming virulence factor.

The Journal of Biological Chemistry
Vijay PancholiHong Jin

Abstract

Cell division and cell wall synthesis are closely linked complex phenomena and play a crucial role in the maintenance and regulation of bacterial virulence. Eukaryotic-type Ser/Thr kinases reported in prokaryotes, including that in group A Streptococcus (GAS) (Streptococcus pyogenes Ser/Thr kinase (SP-STK)), regulate cell division, growth, and virulence. The mechanism of this regulation is, however, unknown. In this study, we demonstrated that SP-STK-controlled cell division is mediated under the positive regulation of secretory protein that possesses a cysteine and histidine-dependent aminohydrolases/peptidases (CHAP) domain with functionally active cell wall hydrolase activity (henceforth named as CdhA (CHAP-domain-containing and chain-forming cell wall hydrolase). Deletion of the CdhA-encoding gene resulted in severe cell division and growth defects in GAS mutants. The mutant expressing the truncated CdhA (devoid of the CHAP domain), although displayed no such defects, it became attenuated for virulence in mice and highly susceptible to cell wall-acting antibiotics, as observed for the mutant lacking CdhA. When CdhA was overexpressed in the wild-type GAS as well as in heterologous strains, Escherichia coli and Staphylococcus...Continue Reading

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Citations

Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lok-To ShamMalcolm E Winkler
Sep 16, 2011·The Journal of Biological Chemistry·Shivani AgarwalVijay Pancholi
Jan 21, 2012·The Journal of Biological Chemistry·Shivani AgarwalVijay Pancholi
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Sandro F F PereiraJonathan Dworkin
Feb 26, 2013·BMC Microbiology·Mario A MartinezSubramanian Dhandayuthapani
Jan 12, 2013·Current Protein & Peptide Science·Alessia RuggieroRita Berisio
Jul 30, 2011·Future Microbiology·Kellie Burnside, Lakshmi Rajagopal
Jan 10, 2012·Current Opinion in Microbiology·Kellie Burnside, Lakshmi Rajagopal
Jul 16, 2013·Environmental Microbiology·Orietta MassiddaWaldemar Vollmer
Oct 30, 2016·Frontiers in Cellular and Infection Microbiology·Rebecca J EdgarNatalia Korotkova
Jun 14, 2020·Applied and Environmental Microbiology·Jhonatan A Hernandez-ValdesOscar P Kuipers

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