PMID: 11342166May 9, 2001Paper

Streptolysin O: the C-terminal, tryptophan-rich domain carries functional sites for both membrane binding and self-interaction but not for stable oligomerization

Biochimica Et Biophysica Acta
S Weis, Michael Palmer

Abstract

Streptolysin O belongs to the class of thiol-activated toxins, which are single chain, four-domain proteins that bind to membranes containing cholesterol and then assemble to form large oligomeric pores. Membrane binding involves a conserved tryptophan-rich sequence motif located within the C-terminally located domain 4. In contrast, sites involved in oligomerization and pore formation have been assigned to domains 1 and 3, respectively. We here examined the functional properties of domain 4, which was recombinantly expressed with an N-terminal histidine tag for purification and an additional cysteine residue for covalent labeling. The fluorescently labeled fragment readily bound to membranes, but it did not form oligomers nor lyse cell membranes. Moreover, the labeled fragment did not detectably become incorporated into hybrid oligomers when combined with lytically active full-length toxin. However, when present in large excess over the active toxin, the domain 4 fragment effected reduction of hemolytic activity and of functional pore size, which indicates interference with oligomerization of the lytically active species. Our findings support the notion that domain 4 of the streptolysin O molecule may fold autonomously, is ess...Continue Reading

References

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Jan 1, 1977·Methods in Enzymology·G R Stark
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
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Citations

Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·M Palmer
Jan 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Galina PolekhinaMichael W Parker
May 23, 2003·Infection and Immunity·Jeffrey G ShannonRichard F Rest
Sep 24, 2005·Infection and Immunity·Rodney K Tweten
Mar 27, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lisa PokrajacMichael Palmer
Aug 5, 2010·Journal of Structural Biology·J Robin HarrisMichael Palmer
Aug 16, 2005·Cellular Microbiology·Nelson O GekaraSiegfried Weiss
Jun 16, 2006·FEMS Microbiology Reviews·Samer Kayal, Alain Charbit
Jul 1, 2016·Future Microbiology·Tobias TenenbaumChristian Schwerk
Sep 11, 2004·FEMS Microbiology Letters·Michael Palmer
Nov 16, 2004·Nature Structural & Molecular Biology·Kara S GiddingsRodney K Tweten
Jun 17, 2020·Expert Review of Anti-infective Therapy·Shamshul Ansari, Yoshio Yamaoka
Mar 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Kaiming ZhangWah Chiu
Dec 18, 2020·Frontiers in Microbiology·Guizhen WangTianqi Fang
May 17, 2006·Anaerobe·Yoshiko Ohno-IwashitaShintaro Iwashita
Sep 25, 2007·Microbes and Infection·Kachiko SekiyaHideaki Nagamune

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