Streptomyces as host for recombinant production of Mycobacterium tuberculosis proteins

Tuberculosis
Carlos VallínJ Anné

Abstract

The 45/47 kDa APA protein (Rv1860) of Mycobacterium tuberculosis was produced by Streptomyces lividans. The recombinant protein could be recovered from the culture medium of an S. lividans clone containing the apa gene under control of the promoter and signal sequence of the Streptomyces coelicolor agarase gene. The recombinant protein production was further scaled-up using fermentation conditions. The APA protein was subsequently purified from the culture supernatant by means of immunochromatography. About 80 mg of recombinant protein were obtained per liter of culture media. In vivo tests with the APA protein purified from S. lividans TK24/pRGAPA1 revealed that the recombinant protein was antigenic and could induce high titers of specific antibodies in the mouse biological model. Results obtained concerning heterologous production of APA, its immunogenic and antigenic capacity, demonstrated the potential of S. lividans as a valuable host for the production of recombinant proteins from M. tuberculosis.

Citations

Mar 5, 2009·Future Microbiology·Kristof Vrancken, Jozef Anné
May 8, 2008·Applied Microbiology and Biotechnology·Margarita DíazRamón Santamaría
Oct 11, 2014·Protein Science : a Publication of the Protein Society·Ghader Bashiri, Edward N Baker
Jul 23, 2011·Journal of Biotechnology·Jozef AnnéKristel Bernaerts
Sep 26, 2020·Frontiers in Microbiology·Francesca BeriniElisa Binda

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