Streptomyces-derived actinomycin D inhibits biofilm formation by Staphylococcus aureus and its hemolytic activity

Biofouling
Jin-Hyung LeeJintae Lee

Abstract

Staphylococcus aureus is a versatile human pathogen that produces diverse virulence factors, and its biofilm cells are difficult to eradicate due to their inherent ability to tolerate antibiotics. The anti-biofilm activities of the spent media of 252 diverse endophytic microorganisms were investigated using three S. aureus strains. An attempt was made to identify anti-biofilm compounds in active spent media and to assess their anti-hemolytic activities and hydrophobicities in order to investigate action mechanisms. Unlike other antibiotics, actinomycin D (0.5 μg ml(-1)) from Streptomyces parvulus significantly inhibited biofilm formation by all three S. aureus strains. Actinomycin D inhibited slime production in S. aureus and it inhibited hemolysis by S. aureus and caused S. aureus cells to become less hydrophobic, thus supporting its anti-biofilm effect. In addition, surface coatings containing actinomycin D prevented S. aureus biofilm formation on glass surfaces. Given these results, FDA-approved actinomycin D warrants further attention as a potential antivirulence agent against S. aureus infections.

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Citations

Nov 14, 2019·Current Pharmaceutical Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Oct 23, 2019·Frontiers in Microbiology·Hema Bhagavathi Sarveswari, Adline Princy Solomon
Aug 2, 2020·Archives of Microbiology·Hisham A AbbasAmira M El-Ganiny
Aug 10, 2018·International Journal of Biometeorology·Michele Antonelli, Davide Donelli
Jul 10, 2019·Frontiers in Microbiology·Janette V PhamYeo Joon Yoon
Jul 17, 2021·Journal of Natural Products·Ziwei YaoJianhua Ju
Nov 14, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Amira M El-GaninyHisham A Abbas

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Datasets Mentioned

BETA
EB120060
KJ200636.1

Methods Mentioned

BETA
PCR
confocal laser microscopy

Software Mentioned

ImageJ
Elements C
COMSTAT
BLAST
NIS

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