Streptomyces Differentiation in Liquid Cultures as a Trigger of Secondary Metabolism

Antibiotics
Angel Manteca, Paula Yagüe

Abstract

Streptomyces is a diverse group of gram-positive microorganisms characterised by a complex developmental cycle. Streptomycetes produce a number of antibiotics and other bioactive compounds used in the clinic. Most screening campaigns looking for new bioactive molecules from actinomycetes have been performed empirically, e.g., without considering whether the bacteria are growing under the best developmental conditions for secondary metabolite production. These screening campaigns were extremely productive and discovered a number of new bioactive compounds during the so-called "golden age of antibiotics" (until the 1980s). However, at present, there is a worrying bottleneck in drug discovery, and new experimental approaches are needed to improve the screening of natural actinomycetes. Streptomycetes are still the most important natural source of antibiotics and other bioactive compounds. They harbour many cryptic secondary metabolite pathways not expressed under classical laboratory cultures. Here, we review the new strategies that are being explored to overcome current challenges in drug discovery. In particular, we focus on those aimed at improving the differentiation of the antibiotic-producing mycelium stage in the laboratory.

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Citations

Jan 11, 2019·Applied Microbiology and Biotechnology·Carlos Barreiro, Miriam Martínez-Castro
Aug 1, 2019·Antibiotics·Yvonne Mast, Evi Stegmann
Jan 20, 2019·Antibiotics·David Gómez-RíosRigoberto Ríos-Estepa
Nov 30, 2018·Microbial Cell Factories·Ramsés A Gamboa-SuasnavartMauricio A Trujillo-Roldán
Apr 21, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Buse CinarSedef Tunca
Dec 19, 2019·Journal of Molecular Microbiology and Biotechnology·Arie Van WierenSudipta Majumdar

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