Streptomyces linear plasmids that contain a phage-like, centrally located, replication origin

Molecular Microbiology
P C ChangStanley N Cohen

Abstract

Unlike previously studied linear replicons containing 5' DNA termini covalently bound to protein, pSLA2, a 17 kb linear plasmid of Streptomyces rochei, initiates replication internally rather than at the telomeres (Chang and Cohen, 1994). Here we identify and characterize the replication origin of pSLA2, showing that it contains a series of direct repeats (iterons) within a centrally located gene encoding an essential DNA-binding protein (Rep1); a second essential protein (Rep2), which resembles prokaryotic DNA helicases and has ATPase activity stimulated by single-stranded DNA, is expressed from the same transcript. A 430 bp locus separated by almost 2 kb from the iterons of the origin specifies an as yet undefined additional function required in cis for plasmid replication. pSCL, a 12 kb linear plasmid of Streptomyces clavuligerus, contains, near the centre of the plasmid, a region configured like the pSLA2 origin. The replication regions of pSLA2 and pSCL, which are capable of propagating plasmid DNA in either a circular or linear form (Shiffman and Cohen, 1992; Chang and Cohen, 1994) resemble those of temperate bacteriophages of the Enterobacteriacae and Bacillus. Our observations suggest that Streptomyces linear plasmids m...Continue Reading

Citations

May 10, 2000·FEMS Microbiology Letters·J N Volff, J Altenbuchner
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Jul 26, 2002·Molecular Microbiology·Zhongjun Qin, Stanley N Cohen
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mario MencíaMargarita Salas
May 16, 2008·Nucleic Acids Research·Hsiu-Hui TsaiCarton W Chen
Sep 8, 2006·Applied and Environmental Microbiology·Ran ZhangZhongjun Qin
Jul 6, 2010·Applied and Environmental Microbiology·Ran ZhangZhongjun Qin
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