Streptomycin resistance-aided genome shuffling to improve doramectin productivity of Streptomyces avermitilis NEAU1069

Journal of Industrial Microbiology & Biotechnology
Ji ZhangWen-Sheng Xiang

Abstract

Genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. In this study, a strategy of incorporating streptomycin resistance screening into genome shuffling (GS-SR) was applied for rapid improvement of doramectin production by Streptomyces avermitilis NEAU1069. The starting mutant population was generated through treatment of the spores with N-methyl-N'-nitro-N-nitrosoguanidine and ultraviolet (UV) irradiation, respectively, and five mutants with higher productivity of doramectin were selected as starting strains for GS-SR. Finally, a genetically stable strain F4-137 was obtained and characterized to be able to yield 992 ± 4.4 mg/l doramectin in a shake flask, which was 7.3-fold and 11.2-fold higher than that of the starting strain UV-45 and initial strain NEAU1069, respectively. The doramectin yield by F4-137 in a 50-l fermentor reached 930.3 ± 3.8 mg/l. Furthermore, the factors associated with the improved doramectin yield were investigated and the results suggested that mutations in ribosomal protein S12 and the enhanced production of cyclohexanecarboxylic coenzyme A may contribute to the improved performance of the shuffled strains. The random amplified po...Continue Reading

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Citations

Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Aug 12, 2014·Applied Microbiology and Biotechnology·Nguyen Huy ThuanJae Kyung Sohng
May 15, 2015·Bioprocess and Biosystems Engineering·Yong-Peng ZhouLei Tang
Feb 20, 2018·Bioprocess and Biosystems Engineering·Qian-Qian TongJian-Ming Yao
May 16, 2014·Applied Biochemistry and Biotechnology·Qingling WangXin-Le Liang
Oct 24, 2019·World Journal of Microbiology & Biotechnology·Ping YuTingting Yan

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