Streptozotocin alters glucose transport, connexin expression and endoplasmic reticulum functions in neurons and astrocytes

Neuroscience
Joyshree BiswasSarika Singh

Abstract

The study was undertaken to explore the cell-specific streptozotocin (STZ)-induced mechanistic alterations. STZ-induced rodent model is a well-established experimental model of Alzheimer's disease (AD) and in our previous studies we have established it as an in vitro screening model of AD by employing N2A neuronal cells. Therefore, STZ was selected in the present study to understand the STZ-induced cell-specific alterations by utilizing neuronal N2A and astrocytes C6 cells. Both neuronal and astrocyte cells were treated with STZ at 10, 50, 100 and 1000μM concentrations for 48h. STZ exposure caused significant decline in cellular viability and augmented cytotoxicity of cells involving astrocytes activation. STZ treatment also disrupted the energy metabolism by altered glucose uptake and its transport in both cells as reflected with decreased expression of glucose transporters (GLUT) 1/3. The consequent decrease in ATP level and decreased mitochondrial membrane potential was also observed in both the cells. STZ caused increased intracellular calcium which could cause the initiation of endoplasmic reticulum (ER) stress. Significant upregulation of ER stress-related markers were observed in both cells after STZ treatment. The cellu...Continue Reading

References

Jan 11, 2014·Clinical and Translational Imaging : Reviews in Nuclear Medicine and Molecular Imaging·Lisa Mosconi

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Citations

Dec 21, 2019·Journal of Neural Transmission·Fruzsina BagaméryTamás Tábi
Dec 24, 2019·Bioscience Reports·Jin-Ting HeXin Zhao
Aug 11, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Zhenhong LiuPengwen Wang
Oct 28, 2021·Bulletin of Experimental Biology and Medicine·E E GenrikhsL G Khaspekov

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