Streptozotocin-induced dynamic metabonomic changes in rat biofluids

Journal of Proteome Research
Wenxin XuHuiru Tang

Abstract

Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were induced by the well-known diabetogenic chemical streptozotocin (STZ), using (1)H NMR spectroscopy in conjunction with multivariate data analysis. The results showed that a single intraperitoneal injection of STZ with a moderate dosage (55 mg/kg) induced significant urinary metabonomic changes within 24 h. These changes showed time-dependence and heterogeneity among the treated animals with an animal recovered within 11 days. STZ-induced metabonomic alterations were related to suppression of glycolysis and TCA cycle, promotion of gluconeogenesis and oxidation of amino acids, alterations in metabolisms of basic amino acids associated with diabetic complications, and disruption of lipid metabolism and gut microbiota functions. With diffusion-edited NMR spectral data, we further observed the STZ-induced significant elevation of monounsaturated fatty acids and total unsaturated fatty acids together with reductions in PUFA-t...Continue Reading

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Citations

Jul 24, 2015·Journal of Pharmaceutical and Biomedical Analysis·Jian-Li ChenGuan-Hua Du
Sep 8, 2015·Archives of Biochemistry and Biophysics·Ning LiYulan Wang
Oct 2, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fang LiLintao Han
Apr 1, 2015·Journal of Proteome Research·Lihong ZhouHuiru Tang
Mar 19, 2015·Journal of Proteome Research·Dan LiHuiru Tang
Feb 12, 2015·Journal of Proteome Research·Ning LiHuiru Tang
Sep 4, 2013·Journal of Proteome Research·Yihuang WangHuiru Tang
Mar 25, 2014·Journal of Agricultural and Food Chemistry·Yangfang YeChunlin Wang
Dec 13, 2012·Journal of Proteome Research·Xiaohuo ShiHuiru Tang
Dec 10, 2020·Gut Microbes·Yuan TianAndrew D Patterson
Jun 12, 2013·Journal of Proteome Research·Yanpeng AnHuiru Tang
Jun 9, 2016·Journal of Proteome Research·Junfang WuHuiru Tang

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