Abstract
A promoter which controls expression of the pristinamycin multidrug resistance gene (ptr) in Streptomyces pristinaspiralis could be induced by physiological stresses in both Streptomyces spp. and Escherichia coli. In S. pristinaspiralis, the ptr promoter (Pptr) was induced by pristinamycin I (PI) or pristinamycin II (PII). Streptomyces lividans was adopted as a convenient heterologous host for studies of Pptr regulation since it has no known pristinamycin biosynthetic genes. Two key regulatory features were documented in these studies: many (19 of 70) antibiotics and chemicals with no common targets or structural features induced the Pptr; induction with PI was most efficient during a transition phase when antibiotic biosynthetic genes are switched on. In Streptomyces coelicolor, Pptr activity was similarly inducible by PI and not dependent on sigma factors HrdA, HrdC, or HrdD. In E. coli, Pptr cloned in the bifunctional promoter probe vector pIJ2839 was functional and activated upon entry into stationary phase in the absence of exogenous inducer. Finally, gel-retardation studies demonstrated a Pptr-binding protein in S. lividans (where its activity was PI-inducible), S. coelicolor and S. pristinaespiralis. The fact that this a...Continue Reading
Citations
May 23, 2001·Biotechnology and Bioengineering·A D FreyM Fussenegger
Aug 1, 1997·Electrophoresis·J VohradskýC J Thompson
Mar 21, 1998·Electrophoresis·J Vohradský
Jan 10, 2002·The Journal of Gene Medicine·S MoserM Fussenegger
May 7, 2010·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe, Arnold L Demain
Jul 27, 2002·Journal of Biotechnology·Wilfried Weber, Martin Fussenegger
Apr 1, 1997·Nature Biotechnology·G SezonovM Guérineau
Apr 29, 2008·Applied and Environmental Microbiology·Angel MantecaJesus Sanchez
Aug 12, 2000·Journal of Bacteriology·J VohradskyC J Thompson
Apr 28, 2001·Journal of Bacteriology·P H ViollierC J Thompson
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Steve GrkovicRonald A Skurray
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Rowan P MorrisCharles J Thompson
Dec 13, 2006·Protein Expression and Purification·Saurabh K GargPushpa Agrawal
Jun 6, 2003·Molecular Microbiology·Jana NovotnaCharles J Thompson
Oct 27, 2007·Proteomics·Jiri VohradskyCharles J Thompson
Nov 4, 2000·Nature Biotechnology·M FusseneggerJ E Bailey
Jan 13, 2005·Canadian Journal of Microbiology·Julie Beauséjour, Carole Beaulieu
Oct 26, 2000·The Journal of Biological Chemistry·M FolcherC J Thompson
Dec 1, 1996·Microbiological Reviews·I T PaulsenR A Skurray
Dec 9, 2014·Microbiology Spectrum·Dirk Schnappinger, Sabine Ehrt