Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in mice.

Scientific Reports
Konrad JuczewskiDavid M. Lovinger

Abstract

Manual restriction of head movement, or head-fixation, of awake rodents allows for sophisticated investigation of neural circuits in vivo, that would otherwise be impossible in completely freely moving animals. While it is known that head-fixation induces stress, the scale of this stress and habituation dynamics remain unclear. We used the Mobile HomeCage system (Neurotar Ltd, Finland) where animals have their heads fixed to an aluminum frame but are otherwise freely moving in an ultralight carbon container floating above an air-dispensing base. For 25 consecutive days, mice were head-fixed while standing on the air-lifted platform for 2 h per day and blood samples were taken periodically to measure variation in the stress-related hormone, corticosterone. We showed that the initial increase in corticosterone concentration is followed by a return to control level throughout the days of head-fixed training. We also found a locomotor correlate of this drop. We conducted a battery of stress-sensitive behavioral paradigms in freely-moving mice that revealed minor differences following chronic head-fixation. Finally, we analyzed motor-skill learning in the head-fixed setup with a floating container. We believe that our results may co...Continue Reading

References

Nov 1, 1963·The American Journal of Physiology·V CritchlowH S Lipscomb
Mar 2, 1962·Science·E V EVARTSP R HUTTENLOCHER
Jan 30, 2004·Trends in Genetics : TIG·Eunju SeongMargit Burmeister
Apr 27, 2005·Endocrinology and Metabolism Clinics of North America·Lauren Jacobson
Oct 7, 2006·Behavioural Brain Research·Yann S MineurWim E Crusio
Apr 5, 2007·Nature Protocols·Robert M J Deacon
Sep 25, 2007·Brain, Behavior, and Immunity·Stephanie L BowersRandy J Nelson
Mar 28, 2008·Neuron·Ivan E de AraujoSidney A Simon
Mar 2, 2010·Neuron·Cristopher M Niell, Michael P Stryker
Oct 20, 2010·Somatosensory & Motor Research·Cornelius SchwarzChristian Waiblinger
Dec 7, 2011·Frontiers in Integrative Neuroscience·Selmaan N ChettihJavier F Medina
Mar 13, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lin SuoLin Lu
Nov 28, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Brendan D HareWilliam A Falls
Feb 4, 2014·Psychoneuroendocrinology·Benjamin D SachsMarc G Caron
Feb 13, 2014·PloS One·Zengcai V GuoKarel Svoboda
May 2, 2014·Frontiers in Behavioral Neuroscience·Annemarie ZimprichSabine M Hölter
Jun 13, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Isaac A Youngstrom, Ben W Strowbridge
Jun 23, 2015·Nature Neuroscience·Simon X ChenTakaki Komiyama
Jul 23, 2015·Lab Animal·Ping-Ping TsaiHansjoachim Hackbarth
Oct 22, 2016·Neuroscience and Biobehavioral Reviews·Russell D RomeoVeronica M So
Aug 4, 2016·Journal of Neurophysiology·Mostafa A NashaatMatthew E Larkum
Oct 25, 2016·Physiology & Behavior·Annelisa M Sadler, Sarah J Bailey
Feb 12, 2017·Physiology & Behavior·Udaiyappan JanakiramanGilles J Guillemin
Jul 22, 2017·Journal of Neuroscience Methods·A GiovannucciD Masip
Sep 18, 2017·Neuroscience·Andrew E Papale, Bryan M Hooks
Mar 1, 2018·Frontiers in Neural Circuits·Zhe HanChengyu T Li
May 8, 2018·Physiology & Behavior·Mandakh BekhbatGretchen N Neigh
Dec 5, 2018·BMC Nephrology·Julius E KieswichMuhammad M Yaqoob
Dec 19, 2019·Frontiers in Behavioral Neuroscience·Brittany N KuhnAna-Clara Bobadilla

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Citations

Sep 12, 2020·Journal of Neural Engineering·James K TrevathanKip Ludwig
Apr 7, 2021·Nature Methods·Mathew L RynesSuhasa B Kodandaramaiah
Jul 21, 2021·Microscopy·Alice M StamatakisKunal K Ghosh

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
EthoVision XT
EthoVision
MHC Tracking
MHC Tracking System
MyAssays

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