Stress granule formation is induced by a threshold temperature rather than a temperature difference in Arabidopsis

Journal of Cell Science
Takahiro HamadaIkuko Hara-Nishimura

Abstract

Stress granules, a type of cytoplasmic RNA granule in eukaryotic cells, are induced in response to various environmental stresses, including high temperature. However, how high temperatures induce the formation of these stress granules in plant cells is largely unknown. Here, we characterized the process of stress granule formation in Arabidopsis thaliana by combining live imaging and electron microscopy analysis. In seedlings grown at 22°C, stress granule formation was induced at temperatures above a critical threshold level of 34°C in the absence of transpiration. The threshold temperature was the same, regardless of whether the seedlings were grown at 22°C or 4°C. High-resolution live imaging microscopy revealed that stress granule formation is not correlated with the sizes of pre-existing RNA processing bodies (P-bodies) but that the two structures often associated rapidly. Immunoelectron microscopy revealed a previously unidentified characteristic of the fine structures of Arabidopsis stress granules and P-bodies: the lack of ribosomes and the presence of characteristic electron-dense globular and filamentous structures. These results provide new insights into the universal nature of stress granules in eukaryotic cells.

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Citations

May 17, 2019·Plant & Cell Physiology·Akihiro MatsuiMotoaki Seki
Sep 19, 2020·Current Protocols in Plant Biology·Monika Kosmacz, Aleksandra Skirycz
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Feb 11, 2021·International Journal of Molecular Sciences·Alena NáprstkováDavid Honys
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Max KoppersGinny G Farías
Oct 14, 2020·Developmental Cell·Ryan J EmeneckerLucia C Strader

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